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DNA construction

MultiGreen: A multiplexing architecture for GreenGate cloning

Vincent J. Pennetti, Peter R. LaFayette, Wayne Allen Parrott

published 18 Sep 2024

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Transcriptional control of a stem cell factor nucleostemin in liver regeneration and aging

Xiaoqin Liu, Junying Wang, Fang Li, Nikolai Timchenko, Robert Y. L. Tsai

published 11 Sep 2024

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Heterogeneity in establishment of polyethylene glycol-mediated plasmid transformations for five forest pathogenic Phytophthora species

Erika N. Dort, Richard C. Hamelin

published 10 Sep 2024

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Strategies found not to be suitable for stabilizing high steroid hydroxylation activities of CYP450 BM3-based whole-cell biocatalysts

Carolin Bertelmann, Bruno Bühler

published 06 Sep 2024

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Stable in vitro fluorescence for enhanced live imaging of infection models for Batrachochytrium dendrobatidis

Rebecca J. Webb, Andrea L. Vu, Lee F. Skerratt, Lee Berger, Francisco De Jesús Andino, Jacques Robert

published 29 Aug 2024

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ActVI-ORFA directs metabolic flux towards actinorhodin by preventing intermediate degradation

Xuechen Zhu, Rongbin Wang, Vilja Siitonen, Nemanja Vuksanovic, Nicholas R. Silvaggi, Charles E. Melançon III, Mikko Metsä-Ketelä

published 09 Aug 2024

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A convenient five-segment cassette procedure for DNA insertions coding for novel peptides

Jonathan Filley

published 26 Jul 2024

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STING inhibition enables efficient plasmid-based gene expression in primary vascular cells: A simple and cost-effective transfection protocol

Shuai Yuan, Adam C. Straub

published 11 Jul 2024

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Enhancement of transcription efficiency by TAR-Tat system increases the functional expression of human olfactory receptors

Ichie Ojiro, Hibiki Katsuyama, Ryusei Kaneko, Satoshi Ogasawara, Takeshi Murata, Yuko Terada, Keisuke Ito

published 25 Jun 2024

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Rv0100: An essential acyl carrier protein from M. tuberculosis important in dormancy

Hiten J. Gutka, Jasper Marc G. Bondoc, Ryan Patwell, Shahebraj Khan, Edyta M. Grzelak, Rajendra Goswami, Martin I. Voskuil, Farahnaz Movahedzadeh

published 07 Jun 2024

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Effects of an indole derivative on cell proliferation, transfection, and alternative splicing in production of lentiviral vectors by transient co-transfection

Nataly Carolina Mier, Donald Keith Roper

published 04 Jun 2024

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A novel replication initiation region encoded in a widespread Acinetobacter plasmid lineage carrying a blaNDM-1 gene

Elena Bello-López, Ángeles Pérez-Oseguera, Walter Santos, Miguel Ángel Cevallos

published 31 May 2024

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Identifying widespread and recurrent variants of genetic parts to improve annotation of engineered DNA sequences

Matthew J. McGuffie, Jeffrey E. Barrick

published 28 May 2024

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