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DNA construction

Clade-1 Vap virulence proteins of Rhodococcus equi are associated with the cell surface and support intracellular growth in macrophages

Zeynep Yerlikaya, Raúl Miranda-CasoLuengo, Yuting Yin, Cheng Cheng, Wim G. Meijer

published 06 Jan 2025

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Plasmidome of Salmonella enterica serovar Infantis recovered from surface waters in a major agricultural region for leafy greens in California

Beatriz Quiñones, Bertram G. Lee, Ashley Avilés Noriega, Lisa Gorski

published 30 Dec 2024

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Neuron-associated retroelement-derived protein Arc/Arg3.1 assists in the early stages of alphaherpesvirus infection in human neuronal cells

Hiroko Kobayashi, Mitsuki Yasukochi, Masayuki Horie, Yasuko Orba, Hirofumi Sawa, Kan Fujino, Satoshi Taharaguchi

published 12 Dec 2024

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Evaluating the specificity of flavivirus proteases in Aedes aegypti cells for dengue virus 2-derived cleavage sites

Alexius O. Dingle, Zach N. Adelman

published 03 Dec 2024

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RecombiCraft library construction: A novel method for DNA library cloning and expansion using non-enzymatic single-step DNA recombination and liquid culture

Yuki Kawai-Harada, Mehrsa Mardikoraem, Katherine Lauro, Vasudha Nimmagadda, Quynh Tong, Kayla Bello, Daniel Woldring, Masako Harada

published 02 Dec 2024

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Novel and effective plasmid transfection protocols for functional analysis of genetic elements in human cardiac fibroblasts

Makoto Matsuyama, Takahiro Iwamiya

published 26 Nov 2024

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A cryptic promoter in the exon of HKR1 drives expression of a truncated form of Hkr1 in Saccharomyces cerevisiae

Toshihiro Kondo, Yuna Hosokawa, Ryotaro Ozawa, Shin Kasahara

published 21 Nov 2024

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The Zymoseptoria tritici effector Zt-11 contributes to aggressiveness in wheat

Sujit Jung Karki, Paola Pilo, Colleen Lawless, Nikolaos Mastrodimos, Anna M. M. Tiley, James Burke, Angela Feechan

published 19 Nov 2024

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Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection

Seyed Alireza Mousavi Kahaki, Nayereh Ebrahimzadeh, Hossein Fahimi, Arfa Moshiri

published 14 Nov 2024

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Increased QPCT gene expression by the hepatitis B virus promotes HBV replication

Conghui Zhang, Qingfeng Ma, Wei Wang, Hui Song, Xue Wang, Fengxia Xu, Chengliang Zhu, Xinghui Liu

published 12 Nov 2024

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In vitro one-pot construction of influenza viral genomes for virus particle synthesis based on reverse genetics system

Ryota Tanaka, Kenji Tamao, Mana Ono, Seiya Yamayoshi, Yoshihiro Kawaoka, Masayuki Su’etsugu, Hiroyuki Noji, Kazuhito V. Tabata

published 08 Nov 2024

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Dolichol kinases from yeast, nematode and human can replace each other and exchange their domains creating active chimeric enzymes in yeast

Danguole Ziogiene, Andrius Burdulis, Albertas Timinskas, Ruta Zinkeviciute, Emilija Vasiliunaite, Milda Norkiene, Alma Gedvilaite

published 07 Nov 2024

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The N-terminal activation function AF-1 domain of ERα interacts directly with the C-terminal AF-2-holding ligand-binding domain to recruit the coactivator proteins

Xiaohui Liu, Yutaka Matsuyama, Makiko Sugiyama, Keitaro Suyama, Takeru Nose, Miki Shimohigashi, Yasuyuki Shimohigashi

published 21 Oct 2024

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