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DNA construction

Plasmidome of Salmonella enterica serovar Infantis recovered from surface waters in a major agricultural region for leafy greens in California

Beatriz Quiñones, Bertram G. Lee, Ashley Avilés Noriega, Lisa Gorski

Neuron-associated retroelement-derived protein Arc/Arg3.1 assists in the early stages of alphaherpesvirus infection in human neuronal cells

Hiroko Kobayashi, Mitsuki Yasukochi, [ ... ], Satoshi Taharaguchi

Evaluating the specificity of flavivirus proteases in Aedes aegypti cells for dengue virus 2-derived cleavage sites

Alexius O. Dingle, Zach N. Adelman

RecombiCraft library construction: A novel method for DNA library cloning and expansion using non-enzymatic single-step DNA recombination and liquid culture

Yuki Kawai-Harada, Mehrsa Mardikoraem, [ ... ], Masako Harada

Novel and effective plasmid transfection protocols for functional analysis of genetic elements in human cardiac fibroblasts

Makoto Matsuyama, Takahiro Iwamiya

A cryptic promoter in the exon of HKR1 drives expression of a truncated form of Hkr1 in Saccharomyces cerevisiae

Toshihiro Kondo, Yuna Hosokawa, Ryotaro Ozawa, Shin Kasahara

The Zymoseptoria tritici effector Zt-11 contributes to aggressiveness in wheat

Sujit Jung Karki, Paola Pilo, [ ... ], Angela Feechan

Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection

Seyed Alireza Mousavi Kahaki, Nayereh Ebrahimzadeh, Hossein Fahimi, Arfa Moshiri

Increased QPCT gene expression by the hepatitis B virus promotes HBV replication

Conghui Zhang, Qingfeng Ma, [ ... ], Xinghui Liu

In vitro one-pot construction of influenza viral genomes for virus particle synthesis based on reverse genetics system

Ryota Tanaka, Kenji Tamao, [ ... ], Kazuhito V. Tabata

Dolichol kinases from yeast, nematode and human can replace each other and exchange their domains creating active chimeric enzymes in yeast

Danguole Ziogiene, Andrius Burdulis, [ ... ], Alma Gedvilaite

The N-terminal activation function AF-1 domain of ERα interacts directly with the C-terminal AF-2-holding ligand-binding domain to recruit the coactivator proteins

Xiaohui Liu, Yutaka Matsuyama, [ ... ], Yasuyuki Shimohigashi

A modified CD9 tag for efficient protein delivery via extracellular vesicles

Shojiro Inano, Toshiyuki Kitano

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