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DNA construction

A Comparison of Exogenous Promoter Activity at the ROSA26 Locus Using a PhiC31 Integrase Mediated Cassette Exchange Approach in Mouse ES Cells

Chiann-mun Chen, Jon Krohn, Shoumo Bhattacharya, Benjamin Davies

Inverse Fusion PCR Cloning

Markus Spiliotis

A Method for Selectively Enriching Microbial DNA from Contaminating Vertebrate Host DNA

George R. Feehery, Erbay Yigit, [ ... ], Sriharsa Pradhan

Unlocking Short Read Sequencing for Metagenomics

Sébastien Rodrigue, Arne C. Materna, [ ... ], Sallie W. Chisholm

A New and Improved Host-Independent Plasmid System for RK2-Based Conjugal Transfer

Trine Aakvik Strand, Rahmi Lale, [ ... ], Svein Valla

A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants

Andreas Binder, Jayne Lambert, [ ... ], Martin Parniske

Quantification Bias Caused by Plasmid DNA Conformation in Quantitative Real-Time PCR Assay

Chih-Hui Lin, Yu-Chieh Chen, Tzu-Ming Pan

Plasmid Construction Using Recombination Activity in the Fission Yeast Schizosaccharomyces pombe

Ayako Chino, Kenji Watanabe, Hisao Moriya

High-Throughput Construction of Intron-Containing Hairpin RNA Vectors for RNAi in Plants

Pu Yan, Wentao Shen, [ ... ], Jun Duan

In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR

Faqing Huang, Joseph Rankin Spangler, Allen Yang Huang

Synthesis of Morphinan Alkaloids in Saccharomyces cerevisiae

Elena Fossati, Lauren Narcross, [ ... ], Vincent J. J. Martin

Improved Genome Editing in Human Cell Lines Using the CRISPR Method

Ivan M. Munoz, Piotr Szyniarowski, [ ... ], Christophe Lachaud

Large Genomic Fragment Deletions and Insertions in Mouse Using CRISPR/Cas9

Luqing Zhang, Ruirui Jia, [ ... ], Yaowu Zheng

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