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DNA construction

cONcat: Computational reconstruction of concatenated fragments from long Oxford Nanopore reads

Alexander J. Petri, Mai Thi-Huyen Nguyen, Anjali Rajwar, Erik Benson, Kristoffer Sahlin

published 24 Jul 2025

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MTMR7 regulates human spermatogonial stem cells proliferation and migration via targeting FLNB

Nianchao Zhou, Tiantian Wu, Yi Yu, Wenxin Gao, Bing Jiang, Haoyue Hu, Xiaoyan Huang, Cong Shen, Yibo Wu, Tingting Gao

published 10 Jul 2025

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pET28g: A Golden Gate-compatible pET vector for protein expression in Escherichia coli, validated by production of functional human ACE2

Inês M. Luís, Mariana Parada, João B. Vicente, Isabel A. Abreu

published 07 Jul 2025

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Coli bond: A dual-function encryption system for secure information storage and transmission by microorganisms

Xuefeng Xiao, Yunuo Song, Jingxuan Hu, Jinchen Han, Wanbin Xing, Chang Liu

published 11 Jun 2025

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Success and limitations in adaptation of Fast-TrACC tissue culture-independent transformation in coffee, cotton, and tree tobacco

Boaz Negin, Laura Daniela Martin-Lesmes, Georg Jander

published 15 May 2025

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Promiscuous structural cross-compatibilities between major shell components of Klebsiella pneumoniae bacterial microcompartments

Lucie Barthe, Damien Balestrino, Bessam Azizi, Delphine Dessaux, Vanessa Soldan, Jeremy Esque, Thomas Schiex, Sophie Barbe, Luis F. Garcia-Alles

published 07 May 2025

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Correlation of p53 oligomeric status and its subcellular localization in the presence of the AML-associated NPM mutant

Aleš Holoubek, Dita Strachotová, Kateřina Wolfová, Petra Otevřelova, Sára Belejová, Pavla Röselová, Aleš Benda, Barbora Brodská, Petr Herman

published 07 May 2025

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Use of allele-specific qPCR and PCR-RFLP analysis for rapid detection of the SARS-CoV-2 variants in Tunisia: A cheap flexible approach adapted for developing countries

Wajdi Ayadi, Fahmi Smaoui, Saba Gargouri, Sana Ferjani, Zaineb Hamzaoui, Awatef Taktak, Amel Chtourou, Houda Skouri-Gargouri, Azza Hadj Sassi, Mouna Ben Sassi, Sameh Trabelsi, Ali Gargouri, Ilhem Boutiba-Ben Boubaker, Héla Karray-Hakim, Raja Mokdad-Gargouri, Lamia Feki-Berrajah

published 05 May 2025

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Expression profiling and transcriptional regulation of the SRS transcription factor gene family of common bean (Phaseolus vulgaris) in symbiosis with Rhizobium etli

Litzy Ayra, Gladys Jiménez-Nopala, Gabriela Guerrero, Sara Isabel Fuentes, Alfonso Leija, Mario Ramírez, Georgina Hernández

published 02 May 2025

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A mix-and-match reverse genetics system for evaluating genetic determinants of orthobunyavirus neurological disease

Heini M. Miettinen, Matthew J. Abbott, Alyssa B. Evans

published 28 Apr 2025

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Repression of ctrA and chpT by a transcriptional regulator of the Xre family that is expressed by RpoN3 and its cognate activator protein in Cereibacter sphaeroides

Benjamín Vega-Baray, José Hernández-Valle, Sebastián Poggio, Laura Camarena

published 15 Apr 2025

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An SDS-NaOH-based method to isolate genome of recombinant adeno-associated virus vectors for physical titer measurement

Xiangying Zhu, Keying Yang, Jinyan Xie, Xilin Feng, Tao Wu, Mengjun Hu, Haijian Wang, Chenghui Yu, Xiaomin Yu, Farhid Hemmatzadeh, Liqing Zhu, Linhua Zhang

published 03 Apr 2025

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PGAP3 is expressed at increased levels in asthmatic ASM and is associated with increased ASM proliferation, contractility and expression of GATA3 and ALOX5

Eric Leslie, Marina Miller, Allison Lafuze, Sofya Svyatskaya, Gil-Soon Choi, Joshua L. Kennedy, Yung-An Huang, Taylor A. Doherty, David H. Broide

published 25 Mar 2025

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