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DNA construction

The plasmid-encoded transcription factor ArdK contributes to the repression of the IMP-6 metallo-β-lactamase gene bla_IMP-6, leading to a carbapenem-susceptible phenotype in the bla_IMP-6-positive Escherichia coli strain A56-1S

Takaya Segawa, Tsuyoshi Sekizuka, [ ... ], Makoto Kuroda

Rapid and highly-specific generation of targeted DNA sequencing libraries enabled by linking capture probes with universal primers

Joel Pel, Amy Leung, [ ... ], Andre Marziali

Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test

Yong Qi, Yinxiu Shao, [ ... ], Yuexi Li

De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor

Laura Marín, Ignacio Gutiérrez-del-Río, [ ... ], Felipe Lombó

RepB C-terminus mutation of a pRi-repABC binary vector affects plasmid copy number in Agrobacterium and transgene copy number in plants

Zarir Vaghchhipawala, Sharon Radke, [ ... ], Xudong Ye

The “speed gene” effect of myostatin arises in Thoroughbred horses due to a promoter proximal SINE insertion

Mary F. Rooney, Emmeline W. Hill, Vincent P. Kelly, Richard K. Porter

Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha

Shigeo S. Sugano, Ryuichi Nishihama, [ ... ], Takayuki Kohchi

A simple approach for multi-targeted shRNA-mediated inducible knockdowns using Sleeping Beauty vectors

Severin Fink, Laurens Zugelder, [ ... ], Thorsten Stühmer

Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection

Gabriela P. Martino, Cristian E. Perez, Christian Magni, Víctor S. Blancato

Type II restriction modification system in Ureaplasma parvum OMC-P162 strain

Heng Ning Wu, Yukiko Nakura, [ ... ], Itaru Yanagihara

Desiccation tolerance in Acinetobacter baumannii is mediated by the two-component response regulator BfmR

John M. Farrow III, Greg Wells, Everett C. Pesci

Simplified inducible system for Trypanosoma brucei

Gabriela T. Niemirowicz, Juan J. Cazzulo, Vanina E. Álvarez, León A. Bouvier

Using a phiC31 “Disintegrase” to make new attP sites in the Drosophila genome at locations showing chromosomal position effects

Mukesh Maharjan, Robert K. Maeda, François Karch, Craig M. Hart

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