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DNA construction

PepN is a non-essential, cell wall-localized protein that contributes to neutrophil elastase-mediated killing of Streptococcus pneumoniae

Charmaine N. Nganje, Scott A. Haynes, Christine M. Qabar, Rachel C. Lent, Elsa N. Bou Ghanem, Mara G. Shainheit

published 01 Feb 2019

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Interaction between the transmembrane domains of Sho1 and Opy2 enhances the signaling efficiency of the Hog1 MAP kinase cascade in Saccharomyces cerevisiae

Tomomi Takayama, Katsuyoshi Yamamoto, Haruo Saito, Kazuo Tatebayashi

published 25 Jan 2019

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Functional screening for triclosan resistance in a wastewater metagenome and isolates of Escherichia coli and Enterococcus spp. from a large Canadian healthcare region

Andrew Cameron, Ruth Barbieri, Ron Read, Deirdre Church, Emelia H. Adator, Rahat Zaheer, Tim A. McAllister

published 24 Jan 2019

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Mutant T4 DNA polymerase for easy cloning and mutagenesis

Ruhu Qi, Gottfried Otting

published 23 Jan 2019

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CRISPR-Cas9 In Situ engineering of subtilisin E in Bacillus subtilis

Marcus A. Price, Rita Cruz, Scott Baxter, Franck Escalettes, Susan J. Rosser

published 07 Jan 2019

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Cowpea chlorotic mottle bromovirus replication proteins support template-selective RNA replication in Saccharomyces cerevisiae

Bryan S. Sibert, Amanda K. Navine, Janice Pennington, Xiaofeng Wang, Paul Ahlquist

published 26 Dec 2018

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The spermidine acetyltransferase SpeG regulates transcription of the small RNA rprA

Linda I. Hu, Ekaterina V. Filippova, Joseph Dang, Sergii Pshenychnyi, Jiapeng Ruan, Olga Kiryukhina, Wayne F. Anderson, Misty L. Kuhn, Alan J. Wolfe

published 18 Dec 2018

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Nab3’s localization to a nuclear granule in response to nutrient deprivation is determined by its essential prion-like domain

Travis J. Loya, Thomas W. O’Rourke, William C. Simke, Joshua B. Kelley, Daniel Reines

published 17 Dec 2018

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Expression of the sRNAs CrcZ and CrcY modulate the strength of carbon catabolite repression under diazotrophic or non-diazotrophic growing conditions in Azotobacter vinelandii

Marcela Martínez-Valenzuela, Josefina Guzmán, Soledad Moreno, Carlos Leonel Ahumada-Manuel, Guadalupe Espín, Cinthia Núñez

published 13 Dec 2018

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The plasmid-encoded transcription factor ArdK contributes to the repression of the IMP-6 metallo-β-lactamase gene bla_IMP-6, leading to a carbapenem-susceptible phenotype in the bla_IMP-6-positive Escherichia coli strain A56-1S

Takaya Segawa, Tsuyoshi Sekizuka, Satowa Suzuki, Keigo Shibayama, Mari Matsui, Makoto Kuroda

published 11 Dec 2018

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Rapid and highly-specific generation of targeted DNA sequencing libraries enabled by linking capture probes with universal primers

Joel Pel, Amy Leung, Wendy W. Y. Choi, Milenko Despotovic, W. Lloyd Ung, Gosuke Shibahara, Laura Gelinas, Andre Marziali

published 05 Dec 2018

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Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test

Yong Qi, Yinxiu Shao, Jixian Rao, Wanpeng Shen, Qiong Yin, Xiaoling Li, Hongxia Chen, Jiameng Li, Wenwen Zeng, Shulong Zheng, Suyun Liu, Yuexi Li

published 26 Nov 2018

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De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor

Laura Marín, Ignacio Gutiérrez-del-Río, Rodrigo Entrialgo-Cadierno, Claudio J. Villar, Felipe Lombó

published 15 Nov 2018

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