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DNA construction

An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue

Jiankang Jin, Yan Xu, Longfei Huo, Lang Ma, Ailing W. Scott, Melissa Pool Pizzi, Yuan Li, Ying Wang, Xiaodan Yao, Shumei Song, Jaffer A. Ajani

published 13 Feb 2020

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AMPKα Subunit Ssp2 and Glycogen Synthase Kinases Gsk3/Gsk31 are involved in regulation of sterol regulatory element-binding protein (SREBP) activity in fission yeast

Hao Miao, Qiannan Liu, Guanglie Jiang, Wen Zhang, Kun Liu, Xiang Gao, Yujie Huo, Si Chen, Toshiaki Kato, Norihiro Sakamoto, Takayoshi Kuno, Yue Fang

published 13 Feb 2020

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Hydrodynamics-based liver transfection achieves gene silencing of CB1 using short hairpin RNA plasmid in cirrhotic rats

Adriana Díaz-Rivera, Alejandra Meza-Ríos, Victoria Chagoya de Sánchez, Gabriela Velasco-Loyden, Leonel García-Benavides, Luis F. Jave-Suarez, Hugo Christian Monroy-Ramirez, Arturo Santos-García, Juan Armendáriz-Borunda, Ana Sandoval-Rodríguez

published 13 Feb 2020

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Very rapid cloning, expression and identifying specificity of T-cell receptors for T-cell engineering

Shan Zong, Tiejuan Mi, Leo G. Flores II, Amir Alpert, Simon Olivares, Krina Patel, Sourindra Maiti, George Mcnamara, Laurence J. N. Cooper, Hiroki Torikai

published 10 Feb 2020

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Improved cell-specificity of adeno-associated viral vectors for medullary thyroid carcinoma using calcitonin gene regulatory elements

Hazel C. Levy, Danielle Hulvey, Laura Adamson-Small, Natacha Jn-Simon, Victor Prima, Scott Rivkees, Jacqueline A. Hobbs

published 06 Feb 2020

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Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen

Tyler W. McCullock, David M. MacLean, Paul J. Kammermeier

published 05 Feb 2020

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Jmjd1c is dispensable for healthy adult hematopoiesis and Jak2^V617F-driven myeloproliferative disease initiation in mice

Hans F. Staehle, Johannes Heinemann, Albert Gruender, Anne M. Omlor, Heike Luise Pahl, Jonas Samuel Jutzi

published 04 Feb 2020

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Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing

Chang-ye Hui, Yan Guo, Lisa Liu, Hao-qu Zheng, Chao-xian Gao, Wen Zhang

published 30 Jan 2020

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Insight into the resilience and susceptibility of marine bacteria to T6SS attack by Vibrio cholerae and Vibrio coralliilyticus

Ryan Guillemette, Blake Ushijima, Mihika Jalan, Claudia C. Häse, Farooq Azam

published 28 Jan 2020

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Gene expression noise in a complex artificial toxin expression system

Alexandra Goetz, Andreas Mader, Benedikt von Bronk, Anna S. Weiss, Madeleine Opitz

published 21 Jan 2020

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Liganded T3 receptor β2 inhibits the positive feedback autoregulation of the gene for GATA2, a transcription factor critical for thyrotropin production

Naoko Hirahara, Hiroko Misawa Nakamura, Shigekazu Sasaki, Akio Matsushita, Kenji Ohba, Go Kuroda, Yuki Sakai, Shinsuke Shinkai, Hiroshi Haeno, Takuhiro Nishio, Shuichi Yoshida, Yutaka Oki, Takafumi Suda

published 15 Jan 2020

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Potentiation of curing by a broad-host-range self-transmissible vector for displacing resistance plasmids to tackle AMR

Alessandro Lazdins, Anand Prakash Maurya, Claire E. Miller, Muhammad Kamruzzaman, Shuting Liu, Elton R. Stephens, Georgina S. Lloyd, Mona Haratianfar, Melissa Chamberlain, Anthony S. Haines, Jan-Ulrich Kreft, Mark. A. Webber, Jonathan Iredell, Christopher M. Thomas

published 15 Jan 2020

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Repository-based plasmid design

Joshua J. Timmons, Doug Densmore

published 09 Jan 2020

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