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DNA construction

Pulse-Field capillary electrophoresis of repeat-primed PCR amplicons for analysis of large repeats in Spinocerebellar Ataxia Type 10

Vera Hashem, Anjana Tiwari, Brittani Bewick, Helio A. G. Teive, Mariana Moscovich, Birgitt Schüele, Khalaf Bushara, Matt Bower, Astrid Rasmussen, Yu-Chih Tsai, Tyson Clark, Karen McFarland, Tetsuo Ashizawa

published 11 Mar 2020

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Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus

Ahmad E. C. Ibrahim, Craig J. van Dolleweerd, Pascal M. W. Drake, Julian K-C. Ma

published 05 Mar 2020

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KLF4 is required for suppression of histamine synthesis by polyamines during bone marrow-derived mast cell differentiation

Kazuhiro Nishimura, Moemi Okamoto, Rina Shibue, Toshio Mizuta, Toru Shibayama, Tetsuhiko Yoshino, Teruki Murakami, Masashi Yamaguchi, Satoshi Tanaka, Toshihiko Toida, Kazuei Igarashi

published 26 Feb 2020

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Manipulating the type VI secretion system spike to shuttle passenger proteins

Sarah Wettstadt, Alain Filloux

published 26 Feb 2020

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An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue

Jiankang Jin, Yan Xu, Longfei Huo, Lang Ma, Ailing W. Scott, Melissa Pool Pizzi, Yuan Li, Ying Wang, Xiaodan Yao, Shumei Song, Jaffer A. Ajani

published 13 Feb 2020

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AMPKα Subunit Ssp2 and Glycogen Synthase Kinases Gsk3/Gsk31 are involved in regulation of sterol regulatory element-binding protein (SREBP) activity in fission yeast

Hao Miao, Qiannan Liu, Guanglie Jiang, Wen Zhang, Kun Liu, Xiang Gao, Yujie Huo, Si Chen, Toshiaki Kato, Norihiro Sakamoto, Takayoshi Kuno, Yue Fang

published 13 Feb 2020

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Hydrodynamics-based liver transfection achieves gene silencing of CB1 using short hairpin RNA plasmid in cirrhotic rats

Adriana Díaz-Rivera, Alejandra Meza-Ríos, Victoria Chagoya de Sánchez, Gabriela Velasco-Loyden, Leonel García-Benavides, Luis F. Jave-Suarez, Hugo Christian Monroy-Ramirez, Arturo Santos-García, Juan Armendáriz-Borunda, Ana Sandoval-Rodríguez

published 13 Feb 2020

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Very rapid cloning, expression and identifying specificity of T-cell receptors for T-cell engineering

Shan Zong, Tiejuan Mi, Leo G. Flores II, Amir Alpert, Simon Olivares, Krina Patel, Sourindra Maiti, George Mcnamara, Laurence J. N. Cooper, Hiroki Torikai

published 10 Feb 2020

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Improved cell-specificity of adeno-associated viral vectors for medullary thyroid carcinoma using calcitonin gene regulatory elements

Hazel C. Levy, Danielle Hulvey, Laura Adamson-Small, Natacha Jn-Simon, Victor Prima, Scott Rivkees, Jacqueline A. Hobbs

published 06 Feb 2020

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Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen

Tyler W. McCullock, David M. MacLean, Paul J. Kammermeier

published 05 Feb 2020

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Jmjd1c is dispensable for healthy adult hematopoiesis and Jak2^V617F-driven myeloproliferative disease initiation in mice

Hans F. Staehle, Johannes Heinemann, Albert Gruender, Anne M. Omlor, Heike Luise Pahl, Jonas Samuel Jutzi

published 04 Feb 2020

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Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing

Chang-ye Hui, Yan Guo, Lisa Liu, Hao-qu Zheng, Chao-xian Gao, Wen Zhang

published 30 Jan 2020

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Insight into the resilience and susceptibility of marine bacteria to T6SS attack by Vibrio cholerae and Vibrio coralliilyticus

Ryan Guillemette, Blake Ushijima, Mihika Jalan, Claudia C. Häse, Farooq Azam

published 28 Jan 2020

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