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DNA construction

The impact of in vitro cultivation on the natural life cycle of the tick-borne relapsing fever spirochete Borrelia turicatae

Aparna Krishnavajhala, Brittany A. Armstrong, Job E. Lopez

Employing lytic phage-mediated horizontal gene transfer in Lactococcus lactis

Barbara Marcelli, Harma Karsens, [ ... ], Jan Kok

A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences

Korbinian N. Kropp, Tim J. Schäufele, [ ... ], Catherine Wölfel

MITF and TFEB cross-regulation in melanoma cells

Josué Ballesteros-Álvarez, Ramile Dilshat, [ ... ], Eiríkur Steingrímsson

Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design

John M. Pryor, Vladimir Potapov, [ ... ], Gregory J. S. Lohman

The critical role of plasma membrane H⁺-ATPase activity in cephalosporin C biosynthesis of Acremonium chrysogenum

Alexander Zhgun, Mariya Dumina, Ayrat Valiakhmetov, Mikhail Eldarov

Genetic incorporation of non-canonical amino acid photocrosslinkers in Neisseria meningitidis: New method provides insights into the physiological function of the function-unknown NMB1345 protein

Hideyuki Takahashi, Naoshi Dohmae, [ ... ], Tatsuo Yanagisawa

Cloning and functional expression of a food-grade circular bacteriocin, plantacyclin B21AG, in probiotic Lactobacillus plantarum WCFS1

Mian Chee Gor, Aida Golneshin, [ ... ], Andrew T. Smith

Tetherin downmodulation by SIVmac Nef lost with the H196Q escape variant is restored by an upstream variant

Blake Schouest, George J. Leslie, James A. Hoxie, Nicholas J. Maness

mCherry fusions enable the subcellular localization of periplasmic and cytoplasmic proteins in Xanthomonas sp.

Michelle Mendonça Pena, Doron Teper, [ ... ], Jesus Aparecido Ferro

An efficient ORF selection system for DNA fragment libraries based on split beta-lactamase complementation

Vaishali Verma, Gopal Joshi, Amita Gupta, Vijay K. Chaudhary

Expression from DIF1-motif promoters of hetR and patS is dependent on HetZ and modulated by PatU3 during heterocyst differentiation

Yaru Du, He Zhang, [ ... ], Xudong Xu

Cloning and expression of a novel α-galactosidase from Lactobacillus amylolyticus L6 with hydrolytic and transgalactosyl properties

Yongtao Fei, WenJuan Jiao, [ ... ], Li Li

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