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DNA construction

Repurposing a microfluidic formulation device for automated DNA construction

Garima Goyal, Nick Elsbree, Michael Fero, Nathan J. Hillson, Gregory Linshiz

published 11 Nov 2020

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Bacterial genome editing by coupling Cre-lox and CRISPR-Cas9 systems

Hualan Liu, David S. Robinson, Zong-Yen Wu, Rita Kuo, Yasuo Yoshikuni, Ian K. Blaby, Jan-Fang Cheng

published 04 Nov 2020

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The Salmonella Typhimurium InvF-SicA complex is necessary for the transcription of sopB in the absence of the repressor H-NS

Luis E. Romero-González, Deyanira Pérez-Morales, Daniel Cortés-Avalos, Edwin Vázquez-Guerrero, Denisse A. Paredes-Hernández, Paulina Estrada-de los Santos, Lourdes Villa-Tanaca, Miguel A. De la Cruz, Víctor H. Bustamante, J. Antonio Ibarra

published 29 Oct 2020

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PCNA antagonizes cohesin-dependent roles in genomic stability

Caitlin M. Zuilkoski, Robert V. Skibbens

published 19 Oct 2020

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The impact of in vitro cultivation on the natural life cycle of the tick-borne relapsing fever spirochete Borrelia turicatae

Aparna Krishnavajhala, Brittany A. Armstrong, Job E. Lopez

published 12 Oct 2020

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Employing lytic phage-mediated horizontal gene transfer in Lactococcus lactis

Barbara Marcelli, Harma Karsens, Mark Nijland, Ruben Oudshoorn, Oscar P. Kuipers, Jan Kok

published 14 Sep 2020

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A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences

Korbinian N. Kropp, Tim J. Schäufele, Martina Fatho, Michael Volkmar, Roland Conradi, Matthias Theobald, Thomas Wölfel, Catherine Wölfel

published 09 Sep 2020

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MITF and TFEB cross-regulation in melanoma cells

Josué Ballesteros-Álvarez, Ramile Dilshat, Valerie Fock, Katrín Möller, Ludwig Karl, Lionel Larue, Margrét Helga Ögmundsdóttir, Eiríkur Steingrímsson

published 03 Sep 2020

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Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design

John M. Pryor, Vladimir Potapov, Rebecca B. Kucera, Katharina Bilotti, Eric J. Cantor, Gregory J. S. Lohman

published 02 Sep 2020

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The critical role of plasma membrane H⁺-ATPase activity in cephalosporin C biosynthesis of Acremonium chrysogenum

Alexander Zhgun, Mariya Dumina, Ayrat Valiakhmetov, Mikhail Eldarov

published 31 Aug 2020

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Genetic incorporation of non-canonical amino acid photocrosslinkers in Neisseria meningitidis: New method provides insights into the physiological function of the function-unknown NMB1345 protein

Hideyuki Takahashi, Naoshi Dohmae, Kwang Sik Kim, Ken Shimuta, Makoto Ohnishi, Shigeyuki Yokoyama, Tatsuo Yanagisawa

published 31 Aug 2020

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Cloning and functional expression of a food-grade circular bacteriocin, plantacyclin B21AG, in probiotic Lactobacillus plantarum WCFS1

Mian Chee Gor, Aida Golneshin, Thi Thu Hao Van, Robert J. Moore, Andrew T. Smith

published 12 Aug 2020

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Tetherin downmodulation by SIVmac Nef lost with the H196Q escape variant is restored by an upstream variant

Blake Schouest, George J. Leslie, James A. Hoxie, Nicholas J. Maness

published 07 Aug 2020

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