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DNA construction

Using runaway replication to express polyhydroxyalkanoic acid (pha) genes from a novel marine bacterium in enteric bacteria: The influence of temperature and phasins on PHA accumulation

Dana Kolibachuk, Benjamin J. Ryder, Edward R. Lyons, Ariel Woolsey, Margaret K. Lopes, Keya Thakkar, Mayelin Pacheco Nunez, Michaela Duquenoy, Nevan R. Valente, Ashley Nieves

published 07 Dec 2022

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Cas9-mediated endogenous plasmid loss in Borrelia burgdorferi

Constantin N. Takacs, Yuko Nakajima, James E. Haber, Christine Jacobs-Wagner

published 28 Nov 2022

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A genetic approach to identify amino acids in Gcn1 required for Gcn2 activation

Susanne Gottfried, Siaosi M. B. M. J. Koloamatangi, Clement Daube, Anja H. Schiemann, Evelyn Sattlegger

published 28 Nov 2022

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The GGDEF-EAL protein CdgB from Azospirillum baldaniorum Sp245, is a dual function enzyme with potential polar localization

Víctor I. Viruega-Góngora, Iris S. Acatitla-Jácome, David Zamorano-Sánchez, Sandra R. Reyes-Carmona, María L. Xiqui-Vázquez, Beatriz Eugenia Baca, Alberto Ramírez-Mata

published 23 Nov 2022

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LupiQuant: A real-time PCR based assay for determining host-to-parasite DNA ratios of Onchocerca lupi and host Canis lupus from onchocercosis samples

Chandler C. Roe, Jennifer Urbanz, Candace Auten, Guilherme G. Verocai, Kelly Upshaw-Bia, Olivia Holiday, Crystal Hepp, Jason W. Sahl

published 21 Nov 2022

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Levy Equilibrium Optimizer algorithm for the DNA storage code set

Jianxia Zhang

published 17 Nov 2022

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Characterization of hepatitis B viral forms from patient plasma using velocity gradient: Evidence for an excess of capsids in fractions enriched in Dane particles

Charlotte Pronier, Jérémy Bomo, Juliette Besombes, Valentine Genet, Syria Laperche, Philippe Gripon, Vincent Thibault

published 16 Nov 2022

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Versatile seamless DNA vector production in E. coli using enhanced phage lambda integrase

Suki Roy, Sabrina Peter, Peter Dröge

published 23 Sep 2022

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High-throughput FastCloning technology: A low-cost method for parallel cloning

Hua Jiang, Fan Meng, Deren Lu, Yanjuan Chen, Guilin Luo, Yuejun Chen, Jun Chen, Cheng Chen, Xi Zhang, Dan Su

published 09 Sep 2022

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An evaluation of fusion partner proteins for paratransgenesis in Asaia bogorensis

Christina Grogan, Marissa Bennett, David J. Lampe

published 01 Sep 2022

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OverFlap PCR: A reliable approach for generating plasmid DNA libraries containing random sequences without a template bias

Artis Linars, Ivars Silamikelis, Dita Gudra, Ance Roga, Davids Fridmanis

published 08 Aug 2022

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Phosphopantetheinyl transferase ClbA contributes to the virulence of avian pathogenic Escherichia coli in meningitis infection of mice

Xia Meng, Yanfei Chen, Peili Wang, Pengpeng Xia, Jinqiu Wang, Mengping He, Chunhong Zhu, Heng Wang, Guoqiang Zhu

published 28 Jul 2022

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Sequence analysis and plasmid mobilization of a 6.6-kb kanamycin resistance plasmid, pSNC3-Kan, from a Salmonella enterica serotype Newport isolate

Chin-Yi Chen, Ly-Huong T. Nguyen, Terence P. Strobaugh Jr.

published 14 Jul 2022

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