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DNA construction

BaEV-pseudotyped lentiviral vectors enable stable CAR expression and cytotoxic function in NK cells

Minji Park, Yuree Lim, Yujung Jo, Dong-Hyeon Jo, Sang-Ki Kim, Hyun-Young Kim, Seung-Hwan Lee, Mijeong Lee, Duck Cho

published 21 May 2026

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Practical guidelines for producing non-replicating canine adenovirus vectors

Denis Omara, Christian Ndekezi, Susan Mugaba, Angella Nakyanzi, Henry Bukenya, Josephine Bwogi, Fortunate Natwijuka, Anne Kapaata, Frank Kato, Drake Byamukama, Mercy Lorna Ayebale, David Patrick Kateete, Jennifer Serwanga, Stephen Cose, Sande James Obondo, Pontiano Kaleebu, Sheila Nina Balinda

published 20 May 2026

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Structural dynamics of the human Orai1 channel revealed by cryo-electron microscopy

Yiming Zhang, Yuan Wang, Jindou Liu, Weiwei Bei, Hongkun Wang, Junli Wang, Lei Chen, Youjun Wang

published 11 May 2026

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The wild, wild west of plasmids: First insights into comparative genomics of Borrelia burgdorferi sensu lato

Sabrina Hepner, Robert E. Rollins, Evangelos Mourkas, Konstantin Kuleshov, Sherwood Casjens, Weigang Qiu, Alexandra Dangel, Dunia Velázquez, Sergey Y. Kovalev, Andreas Wieser, Johannes Hübner, Andreas Sing, Volker Fingerle, Gabriele Margos

published 08 May 2026

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Sequencing complete plasmids on Oxford Nanopore Technologies sequencers using R2C2 and Chopper

Kayla D. Schimke, Christopher Vollmers

published 10 Apr 2026

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Enhancement of RecET-mediated in vivo linear DNA assembly by a xonA mutation

James A. Sawitzke, Nina Costantino, Adriana Castillo Caballero, Ellen Hutchinson, Alessandro Barenghi, Lynn C. Thomason, Donald L. Court

published 03 Apr 2026

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Transcriptional and functional characterization of terpene synthase genes of the aromatic plant Plectranthus hadiensis

Hinano Mizuno, Kenro Tokuhiro, Koichiro Iwai, Yoshikazu Furuta, Akari Nakasone, Hidenori Tanaka, Hiroki Sugimoto

published 23 Mar 2026

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A cluster of acidic residues in the cytoplasmic domain of SARS-CoV-2 Spike is required for virion-incorporation and infectivity

Charlotte A. Stoneham, Rajendra Singh, Amalia De Leon, Petra Tafelmeyer, Francisco Acosta, Angus Fuori, Michael Anderson, Peter W. Ramirez, Hannah S. Schwartzer-Sperber, Satish Pillai, Mary K. Lewinski, John Guatelli

published 12 Mar 2026

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TaqMan qPCR quantification of the Cytb gene for precise determination of camel milk in dairy products

Qin Na, Rendalai Si, Liang Ming, Li Yi, Jing He, Tuyatsetseg Jambal, Rimutu Ji

published 25 Feb 2026

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A new mechanism regulating microglial NLRP3 inflammasome: FMR1 mediates NLRP3 mRNA stability

Qian Deng, Qianqian Bai, Yang Yang, Wenchao Tang, Fangfei Liu, Shumin Zhang, Hao Wang, Zhehua Xing, Chi Zhang, Yanhui Yang, Qizhi Fu, Hua Fan

published 20 Feb 2026

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Application of NMRK immobilized enzymes in the synthesis of NMN

Shan Yang, Ting Xiang, Jinchun Qian, Wei Sun, Wenjun Liu, Nengwen Yi, Zheng-Hong Qin

published 19 Feb 2026

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96 sample parallel acoustic fragmentation for high throughput next generation sequencing library preparation

Marjan Mehrab-Mohseni, Kathlyne Jayne B. Bautista, Yusha Liu, Erika E. Maldonado-Rosado, Haofan Zheng, Matthew J. Niederhuber, Jonathan D. Rosen, Michell D. Carroll, Brian Velasco, James K. Tsuruta, Jinwook Kim, James S. Marron, Perry D. Haaland, Sandeep K. Kasoji, Piotr A. Mieczkowski, Austin J. Hepperla, Paul A. Dayton, Samantha G. Pattenden

published 17 Feb 2026

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Highly efficient production of transgenic rats with long DNA insertions using piggyBac transposase mRNA and piezo-assisted microinjection

Kohtaro Morita, Shunya Ihashi, Eiichi Okamura, Kazuya Goto, Toru Yoshihara, Arata Honda, Masatsugu Ema, Masahide Asano

published 17 Feb 2026

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