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Directed evolution

Complex Structure of Engineered Modular Domains Defining Molecular Interaction between ICAM-1 and Integrin LFA-1

Sungkwon Kang, Chae Un Kim, [ ... ], Moonsoo M. Jin

Fitness Loss and Library Size Determination in Saturation Mutagenesis

Yuval Nov

Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae

Chenlu Zhang, Ligia Acosta-Sampson, Vivian Yaci Yu, Jamie H. D. Cate

Circular Permutation in the Ω-Loop of TEM-1 β-Lactamase Results in Improved Activity and Altered Substrate Specificity

Gurkan Guntas, Manu Kanwar, Marc Ostermeier

An Automated Flow for Directed Evolution Based on Detection of Promiscuous Scaffolds Using Spatial and Electrostatic Properties of Catalytic Residues

Sandeep Chakraborty

SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity

Derek Cerchione, Katherine Loveluck, [ ... ], Barrett E. Steinberg

Fluorinated oil-surfactant mixtures with the density of water: Artificial cells for synthetic biology

Roberto Laos, Steven Benner

Directed Evolution of the Transcriptional Regulator DntR: Isolation of Mutants with Improved DNT-Response

Rosa Lönneborg, Edina Varga, Peter Brzezinski

Directed evolution provides insight into conformational substrate sampling by SrtA

Muna Suliman, Vishaka Santosh, [ ... ], William A. Barton

Modeling Analysis of Signal Sensitivity and Specificity by Vibrio fischeri LuxR Variants

Deanna M. Colton, Eric V. Stabb, Stephen J. Hagen

Computational Design of the β-Sheet Surface of a Red Fluorescent Protein Allows Control of Protein Oligomerization

Timothy M. Wannier, Matthew M. Moore, Yun Mou, Stephen L. Mayo

Directed Evolution of Aminoglycoside Phosphotransferase (3′) Type IIIa Variants That Inactivate Amikacin but Impose Significant Fitness Costs

Joseph R. Kramer, Ichiro Matsumura

Seven N-terminal Residues of a Thermophilic Xylanase Are Sufficient to Confer Hyperthermostability on Its Mesophilic Counterpart

Shan Zhang, Yongzhi He, Haiying Yu, Zhiyang Dong

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