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Directed evolution

Computational Design of the β-Sheet Surface of a Red Fluorescent Protein Allows Control of Protein Oligomerization

Timothy M. Wannier, Matthew M. Moore, Yun Mou, Stephen L. Mayo

published 15 Jun 2015

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In-Frame Amber Stop Codon Replacement Mutagenesis for the Directed Evolution of Proteins Containing Non-Canonical Amino Acids: Identification of Residues Open to Bio-Orthogonal Modification

James A. J. Arpino, Amy J. Baldwin, Adam R. McGarrity, Eric M. Tippmann, D. Dafydd Jones

published 26 May 2015

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Modeling Analysis of Signal Sensitivity and Specificity by Vibrio fischeri LuxR Variants

Deanna M. Colton, Eric V. Stabb, Stephen J. Hagen

published 11 May 2015

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Computational Design of Binding Proteins to EGFR Domain II

Yoon Sup Choi, Soomin Yoon, Kyung-Lock Kim, Jiho Yoo, Parkyong Song, Minsoo Kim, Young-Eun Shin, Won Jun Yang, Jung-eun Noh, Hyun-soo Cho, Sanguk Kim, Junho Chung, Sung Ho Ryu

published 07 Apr 2014

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A High-Throughput Colorimetric Screening Assay for Terpene Synthase Activity Based on Substrate Consumption

Maiko Furubayashi, Mayu Ikezumi, Jun Kajiwara, Miki Iwasaki, Akira Fujii, Ling Li, Kyoichi Saito, Daisuke Umeno

published 28 Mar 2014

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Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

David Gonzalez-Perez, Patricia Molina-Espeja, Eva Garcia-Ruiz, Miguel Alcalde

published 10 Mar 2014

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Seven N-terminal Residues of a Thermophilic Xylanase Are Sufficient to Confer Hyperthermostability on Its Mesophilic Counterpart

Shan Zhang, Yongzhi He, Haiying Yu, Zhiyang Dong

published 30 Jan 2014

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Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution

Vânia Brissos, Nádia Gonçalves, Eduardo P. Melo, Lígia O. Martins

published 27 Jan 2014

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Improving Glyphosate Oxidation Activity of Glycine Oxidase from Bacillus cereus by Directed Evolution

Tao Zhan, Kai Zhang, Yangyan Chen, Yongjun Lin, Gaobing Wu, Lili Zhang, Pei Yao, Zongze Shao, Ziduo Liu

published 05 Nov 2013

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Directed Evolution of Aminoglycoside Phosphotransferase (3′) Type IIIa Variants That Inactivate Amikacin but Impose Significant Fitness Costs

Joseph R. Kramer, Ichiro Matsumura

published 18 Oct 2013

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Fitness Loss and Library Size Determination in Saturation Mutagenesis

Yuval Nov

published 03 Jul 2013

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Complex Structure of Engineered Modular Domains Defining Molecular Interaction between ICAM-1 and Integrin LFA-1

Sungkwon Kang, Chae Un Kim, Xiaoling Gu, Roisin M. Owens, Sarah J. van Rijn, Vanissra Boonyaleepun, Yuxin Mao, Timothy A. Springer, Moonsoo M. Jin

published 30 Aug 2012

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An Automated Flow for Directed Evolution Based on Detection of Promiscuous Scaffolds Using Spatial and Electrostatic Properties of Catalytic Residues

Sandeep Chakraborty

published 11 Jul 2012

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