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Directed evolution

Computational Design of the β-Sheet Surface of a Red Fluorescent Protein Allows Control of Protein Oligomerization

Timothy M. Wannier, Matthew M. Moore, Yun Mou, Stephen L. Mayo

In-Frame Amber Stop Codon Replacement Mutagenesis for the Directed Evolution of Proteins Containing Non-Canonical Amino Acids: Identification of Residues Open to Bio-Orthogonal Modification

James A. J. Arpino, Amy J. Baldwin, [ ... ], D. Dafydd Jones

Modeling Analysis of Signal Sensitivity and Specificity by Vibrio fischeri LuxR Variants

Deanna M. Colton, Eric V. Stabb, Stephen J. Hagen

Computational Design of Binding Proteins to EGFR Domain II

Yoon Sup Choi, Soomin Yoon, [ ... ], Sung Ho Ryu

A High-Throughput Colorimetric Screening Assay for Terpene Synthase Activity Based on Substrate Consumption

Maiko Furubayashi, Mayu Ikezumi, [ ... ], Daisuke Umeno

Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

David Gonzalez-Perez, Patricia Molina-Espeja, Eva Garcia-Ruiz, Miguel Alcalde

Seven N-terminal Residues of a Thermophilic Xylanase Are Sufficient to Confer Hyperthermostability on Its Mesophilic Counterpart

Shan Zhang, Yongzhi He, Haiying Yu, Zhiyang Dong

Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution

Vânia Brissos, Nádia Gonçalves, Eduardo P. Melo, Lígia O. Martins

Improving Glyphosate Oxidation Activity of Glycine Oxidase from Bacillus cereus by Directed Evolution

Tao Zhan, Kai Zhang, [ ... ], Ziduo Liu

Directed Evolution of Aminoglycoside Phosphotransferase (3′) Type IIIa Variants That Inactivate Amikacin but Impose Significant Fitness Costs

Joseph R. Kramer, Ichiro Matsumura

Fitness Loss and Library Size Determination in Saturation Mutagenesis

Yuval Nov

Complex Structure of Engineered Modular Domains Defining Molecular Interaction between ICAM-1 and Integrin LFA-1

Sungkwon Kang, Chae Un Kim, [ ... ], Moonsoo M. Jin

An Automated Flow for Directed Evolution Based on Detection of Promiscuous Scaffolds Using Spatial and Electrostatic Properties of Catalytic Residues

Sandeep Chakraborty

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