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Directed evolution

Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity

Yi Shen, Yingche Chen, [ ... ], Robert E. Campbell

New Molecular Reporters for Rapid Protein Folding Assays

Stéphanie Cabantous, Yvonne Rogers, Thomas C. Terwilliger, Geoffrey S. Waldo

A High-Throughput Colorimetric Screening Assay for Terpene Synthase Activity Based on Substrate Consumption

Maiko Furubayashi, Mayu Ikezumi, [ ... ], Daisuke Umeno

Improved yellow-green split fluorescent proteins for protein labeling and signal amplification

Shuqin Zhou, Siyu Feng, David Brown, Bo Huang

Directed Chemical Evolution with an Outsized Genetic Code

Casey J. Krusemark, Nicolas P. Tilmans, Patrick O. Brown, Pehr B. Harbury

Directed evolution to improve the catalytic efficiency of urate oxidase from Bacillus subtilis

Wenjie Li, Shouteng Xu, [ ... ], Jiong Hong

Computational Design of Binding Proteins to EGFR Domain II

Yoon Sup Choi, Soomin Yoon, [ ... ], Sung Ho Ryu

A Thermostable β-Glucuronidase Obtained by Directed Evolution as a Reporter Gene in Transgenic Plants

Ai-Sheng Xiong, Ri-He Peng, [ ... ], Quan-Hong Yao

In-Frame Amber Stop Codon Replacement Mutagenesis for the Directed Evolution of Proteins Containing Non-Canonical Amino Acids: Identification of Residues Open to Bio-Orthogonal Modification

James A. J. Arpino, Amy J. Baldwin, [ ... ], D. Dafydd Jones

Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution

Vânia Brissos, Nádia Gonçalves, Eduardo P. Melo, Lígia O. Martins

The Molecular Basis for the Broad Substrate Specificity of Human Sulfotransferase 1A1

Ilana Berger, Chen Guttman, [ ... ], Amir Aharoni

Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

David Gonzalez-Perez, Patricia Molina-Espeja, Eva Garcia-Ruiz, Miguel Alcalde

Improving Glyphosate Oxidation Activity of Glycine Oxidase from Bacillus cereus by Directed Evolution

Tao Zhan, Kai Zhang, [ ... ], Ziduo Liu

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