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cDNA libraries

A gene-specific RNA enrichment protocol for nanopore direct-RNA sequencing

Maja Bele Dyrendalsli, Cecilie Løkke, Christer Einvik

published 11 Feb 2026

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Evaluation of metatranscriptomic sequencing protocols to obtain full-length RNA virus genomes from mammalian tissues

Yiqiao Li, Mariana Polychronopoulou, Ine Boonen, Antonios Fikatas, Sophie Gryseels, Anne Laudisoit, Joelle Gouy de Bellocq, Bram Vrancken, Gkikas Magiorkinis, Philippe Lemey, Magda Bletsa

published 30 May 2025

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Reliable RNA-seq analysis from FFPE specimens as a means to accelerate cancer-related health disparities research

Mitchell J. Frederick, Dannelys Perez-Bello, Pedram Yadollahi, Patricia Castro, Alan Frederick, Andrew Frederick, Rashid A. Osman, Fonma Essien, Imelda Yebra, Ashley Hamlin, Thomas J. Ow, Heath D. Skinner, Vlad C. Sandulache

published 21 Apr 2025

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Next-generation sequencing protocol of hematopoietic stem cells (HSCs). Step-by-step overview and troubleshooting guide

Justyna Jarczak, Kamila Bujko, Katarzyna Brzeźniakiewicz-Janus, Mariusz Ratajczak, Magdalena Kucia

published 09 Jan 2025

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Spatial transcriptomic profiling of isolated microregions in tissue sections utilizing laser-induced forward transfer

Kaiqiang Ye, Wanqing Chang, Jitao Xu, Yunxia Guo, Qingyang Qin, Kaitong Dang, Xiaofeng Han, Xiaolei Zhu, Qinyu Ge, Qiannan Cui, Yun Xu, Xiangwei Zhao

published 25 Jul 2024

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A cell competition system with one gene expression from a single-copy gene in one cell

Yoshinori Hasegawa, Megumi Nakano, Tsutomu Hosouchi, Takashi Watanabe, Izumi Yamaguchi, Manabu Nakayama, Osamu Ohara

published 05 Jul 2024

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RNA-seq RNAaccess identified as the preferred method for gene expression analysis of low quality FFPE samples

Kai Song, Emon Elboudwarej, Xi Zhao, Luting Zhuo, David Pan, Jinfeng Liu, Carrie Brachmann, Scott D. Patterson, Oh Kyu Yoon, Marianna Zavodovskaya

published 26 Oct 2023

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A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues

Rose Ruiz Daniels, Richard S. Taylor, Ross Dobie, Sarah Salisbury, James J. Furniss, Emily Clark, Daniel J. Macqueen, Diego Robledo

published 07 Sep 2023

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High throughput isolation of RNA from single-cells within an intact tissue for spatial and temporal sequencing a reality

John Stanley, Akshar Lohith, Lucca Debiaso, Kevan Wang, Minh Ton, Wenwu Cui, Weiwei Gu, Aihua Fu, Nader Pourmand

published 01 Aug 2023

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Pervasive mRNA uridylation in fission yeast is catalysed by both Cid1 and Cid16 terminal uridyltransferases

Lidia Lipińska-Zubrycka, Maciej Grochowski, Jürg Bähler, Michał Małecki

published 23 May 2023

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Optimised protocol for monitoring SARS-CoV-2 in wastewater using reverse complement PCR-based whole-genome sequencing

Harry T. Child, Paul A. O’Neill, Karen Moore, William Rowe, Hubert Denise, David Bass, Matthew J. Wade, Matt Loose, Steve Paterson, Ronny van Aerle, Aaron R. Jeffries

published 14 Apr 2023

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Bioinformatic analysis predicts that ethanol exposure during early development causes alternative splicing alterations of genes involved in RNA post-transcriptional regulation

Camilo Fuentes-Beals, Montserrat Olivares-Costa, María Estela Andrés, Paola A. Haeger, Gonzalo Riadi, Carlos Oliva, Fernando Faunes

published 13 Apr 2023

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A novel eukaryotic RdRP-dependent small RNA pathway represses antiviral immunity by controlling an ERK pathway component in the black-legged tick

Canran Feng, Kyosuke Torimaru, Mandy Yu Theng Lim, Li-Ling Chak, Masami Shiimori, Kosuke Tsuji, Tetsuya Tanaka, Junko Iida, Katsutomo Okamura

published 30 Mar 2023

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