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Biomolecular isolation

Absolute quantification of E. coli virulence and housekeeping genes to determine pathogen loads in enumerated environmental samples

K. B. Hoorzook, T. G. Barnard

An optimized method for RNA extraction from the polyurethane oligomer degrading strain Pseudomonas capeferrum TDA1 growing on aromatic substrates such as phenol and 2,4-diaminotoluene

María José Cárdenas Espinosa, Tabea Schmidgall, [ ... ], Christian Eberlein

Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing

Harutaro Kenmotsu, Emi Takabayashi, [ ... ], Toshihiko Eki

Uncovering the stability status of the reputed reference genes in breast and hepatic cancer cell lines

Gilar Gorji-Bahri, Niloofar Moradtabrizi, Atieh Hashemi

Detection of six soil-transmitted helminths in human stool by qPCR- a systematic workflow

Kristy I. Azzopardi, Myra Hardy, [ ... ], Andrew C. Steer

A workflow for the relative quantification of multiple fish species from oceanic water samples using environmental DNA (eDNA) to support large-scale fishery surveys

Ana Ramón-Laca, Abigail Wells, Linda Park

Protocol for safe, affordable, and reproducible isolation and quantitation of SARS-CoV-2 RNA from wastewater

Monica Trujillo, Kristen Cheung, [ ... ], John J. Dennehy

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation

Maria Jose Lista, Pedro M. Matos, [ ... ], Rocio Teresa Martinez-Nunez

Establishment of a quadruplex real-time PCR assay to distinguish the fungal pathogens Diaporthe longicolla, D. caulivora, D. eres, and D. novem on soybean

Behnoush Hosseini, Ralf T. Voegele, Tobias I. Link

Differential prevalence of pathobionts and host gene polymorphisms in chronic inflammatory intestinal diseases: Crohn’s disease and intestinal tuberculosis

Imteyaz Ahmad Khan, Baibaswata Nayak, [ ... ], Vineet Ahuja

Concatemeric Broccoli reduces mRNA stability and induces aggregates

Marco R. Rink, Marisa A. P. Baptista, [ ... ], Jens B. Bosse

Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles

Tatiana I. Manukhova, Ekaterina A. Evtushenko, [ ... ], Olga V. Karpova

Efficient method for isolation of high-quality RNA from Psidium guajava L. tissues

Paola de Avelar Carpinetti, Vinicius Sartori Fioresi, [ ... ], Marcia Flores da Silva Ferreira

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