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Artificial genetic recombination

Establishment of Cre/LoxP-mediated multifunctional reporter knock-in rats with the CRISPR system

Katsuyuki Nakamura, Sara Ito, Yoshihiro Ohguchi, Toshie Jimbo, Yusaku Wada, Ryota Nakajima, Masanobu Kanou, Kei Yamana, Hiroshi Ueda

published 25 Jun 2025

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Use of allele-specific qPCR and PCR-RFLP analysis for rapid detection of the SARS-CoV-2 variants in Tunisia: A cheap flexible approach adapted for developing countries

Wajdi Ayadi, Fahmi Smaoui, Saba Gargouri, Sana Ferjani, Zaineb Hamzaoui, Awatef Taktak, Amel Chtourou, Houda Skouri-Gargouri, Azza Hadj Sassi, Mouna Ben Sassi, Sameh Trabelsi, Ali Gargouri, Ilhem Boutiba-Ben Boubaker, Héla Karray-Hakim, Raja Mokdad-Gargouri, Lamia Feki-Berrajah

published 05 May 2025

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Development of a lambda Red based system for gene deletion in Chlamydia

Yibing Wang, Robert Suchland, Amy Hua, Steven Carrell, Daniel Rockey, Kevin Hybiske

published 14 Nov 2024

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Hit screening with multivariate robust outlier detection

Hui Sun Leong, Tianhui Zhang, Adam Corrigan, Alessia Serrano, Ulrike Künzel, Niamh Mullooly, Ceri Wiggins, Yinhai Wang, Steven Novick

published 12 Sep 2024

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A statistical simulation model to guide the choices of analytical methods in arrayed CRISPR screen experiments

Chang Sik Kim, Jonathan Cairns, Valentina Quarantotti, Bogumil Kaczkowski, Yinhai Wang, Peter Konings, Xiang Zhang

published 20 Aug 2024

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Non-replicative phage particles delivering CRISPR-Cas9 to target major bla_CTX-M variants

Naiyaphat Nittayasut, Teerapong Yata, Sunisa Chirakul, Navapon Techakriengkrai, Pattrarat Chanchaithong

published 16 May 2024

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Construction and characterization of a hypervesiculation strain of Escherichia coli Nissle 1917

Tomomi Sawabe, Yoshihiro Ojima, Mao Nakagawa, Toru Sawada, Yuhei O. Tahara, Makoto Miyata, Masayuki Azuma

published 02 Apr 2024

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Knockout of CLTC gene reduces but not completely block SFTSV infection

Tiezhu Liu, Jiajia Li, Xueqi Wang, Tao Huang, Wei Wu, Aqian Li, Chuan Li, Xiaoxia Huang, Qin Wang, Dexin Li, Shiwen Wang, Mifang Liang

published 25 Aug 2023

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Droplet digital PCR versus real-time PCR for in-house validation of porcine detection and quantification protocol: An artificial recombinant plasmid approach

Umi Nuraeni, Jekmal Malau, Retno Tri Astuti, Auraga Dewantoro, Dini Apriori, Evellin Dewi Lusiana, Bambang Prasetya

published 14 Jul 2023

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Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli

Riko Shirakawa, Kazuya Ishikawa, Kazuyuki Furuta, Chikara Kaito

published 24 Mar 2023

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Research experimental design for the construction and identification of the pGEX-BCKD-E4A recombinant point-mutant plasmid

Tiannan Zhou, Huixian Wei, Jinjun Wang

published 24 Feb 2023

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Characterization of a new IN-I-PpoI fusion protein and a homology-arm containing transgene cassette that improve transgene expression persistence and 28S rRNA gene-targeted insertion of lentiviral vectors

Alisa Nousiainen, Diana Schenkwein, Seppo Ylä-Herttuala

published 20 Jan 2023

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Preliminary study on the effect of catabolite repression gene knockout on p-nitrophenol degradation in Pseudomonas putida DLL-E4

Shuang Li, Yichao Tang, Lingran Tang, Xuanyu Yan, Jiali Xiao, Huijun Xiang, Qing Wu, Ruqi Yu, Yushi Jin, Jingyu Yu, Nuo Xu, Chu Wu, Shengqin Wang, Chuanhua Wang, Qiongzhen Chen

published 02 Dec 2022

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