Confocal microscopy of Schistosoma mansoni schistosomula illustrates the ease by which dead (propidium iodide-positive, red individuals) and live (fluorescein diacetate-positive, green individuals) parasites can be distinguished using a helminth fluorescent bioassay (HFB) described by Peak et al. (doi:10.1371/journal.pntd.0000759). It is anticipated that the HFB will be useful in objectively measuring schistosome survival during immunological assays (antibody- or complement-mediated killing), RNAi investigations, and drug screening protocols.
Image Credit: Emily Peak
Editorial
The Global Atlas of Helminth Infection: Mapping the Way Forward in Neglected Tropical Disease Control
PLOS Neglected Tropical Diseases: published July 27, 2010 | https://doi.org/10.1371/journal.pntd.0000779
Viewpoints
Mycobacterium ulcerans and Other Mycolactone-Producing Mycobacteria Should Be Considered a Single Species
PLOS Neglected Tropical Diseases: published July 27, 2010 | https://doi.org/10.1371/journal.pntd.0000663
Research Articles
Asymmetric Wolbachia Segregation during Early Brugia malayi Embryogenesis Determines Its Distribution in Adult Host Tissues
PLOS Neglected Tropical Diseases: published July 27, 2010 | https://doi.org/10.1371/journal.pntd.0000758
Development and Validation of a Quantitative, High-Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability
PLOS Neglected Tropical Diseases: published July 27, 2010 | https://doi.org/10.1371/journal.pntd.0000759
Neglected Tropical Diseases outside the Tropics
PLOS Neglected Tropical Diseases: published July 27, 2010 | https://doi.org/10.1371/journal.pntd.0000762
Rift Valley Fever Virus Seroprevalence in Human Rural Populations of Gabon
PLOS Neglected Tropical Diseases: published July 27, 2010 | https://doi.org/10.1371/journal.pntd.0000763
A Curative Immune Profile One Week after Treatment of Indian Kala-Azar Patients Predicts Success with a Short-Course Liposomal Amphotericin B Therapy
PLOS Neglected Tropical Diseases: published July 27, 2010 | https://doi.org/10.1371/journal.pntd.0000764
Phylogeography of Recently Emerged DENV-2 in Southern Viet Nam
PLOS Neglected Tropical Diseases: published July 27, 2010 | https://doi.org/10.1371/journal.pntd.0000766
Randomised Controlled Double-Blind Non-Inferiority Trial of Two Antivenoms for Saw-Scaled or Carpet Viper (Echis ocellatus) Envenoming in Nigeria
PLOS Neglected Tropical Diseases: published July 27, 2010 | https://doi.org/10.1371/journal.pntd.0000767
Health Education through Analogies: Preparation of a Community for Clinical Trials of a Vaccine against Hookworm in an Endemic Area of Brazil
PLOS Neglected Tropical Diseases: published July 20, 2010 | https://doi.org/10.1371/journal.pntd.0000749
The Jacob2 Lectin of the Entamoeba histolytica Cyst Wall Binds Chitin and Is Polymorphic
PLOS Neglected Tropical Diseases: published July 20, 2010 | https://doi.org/10.1371/journal.pntd.0000750
Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana
PLOS Neglected Tropical Diseases: published July 20, 2010 | https://doi.org/10.1371/journal.pntd.0000751
High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi
PLOS Neglected Tropical Diseases: published July 20, 2010 | https://doi.org/10.1371/journal.pntd.0000752
Evaluation of Chikungunya Diagnostic Assays: Differences in Sensitivity of Serology Assays in Two Independent Outbreaks
PLOS Neglected Tropical Diseases: published July 20, 2010 | https://doi.org/10.1371/journal.pntd.0000753
Comparing Diagnostic Accuracy of Kato-Katz, Koga Agar Plate, Ether-Concentration, and FLOTAC for Schistosoma mansoni and Soil-Transmitted Helminths
PLOS Neglected Tropical Diseases: published July 20, 2010 | https://doi.org/10.1371/journal.pntd.0000754
A Tractable Experimental Model for Study of Human and Animal Scabies
PLOS Neglected Tropical Diseases: published July 20, 2010 | https://doi.org/10.1371/journal.pntd.0000756
Emergence of the Asian 1 Genotype of Dengue Virus Serotype 2 in Viet Nam: In Vivo Fitness Advantage and Lineage Replacement in South-East Asia
PLOS Neglected Tropical Diseases: published July 20, 2010 | https://doi.org/10.1371/journal.pntd.0000757
The First Human Epitope Map of the Alphaviral E1 and E2 Proteins Reveals a New E2 Epitope with Significant Virus Neutralizing Activity
PLOS Neglected Tropical Diseases: published July 13, 2010 | https://doi.org/10.1371/journal.pntd.0000739
In Vitro and In Vivo High-Throughput Assays for the Testing of Anti-Trypanosoma cruzi Compounds
PLOS Neglected Tropical Diseases: published July 13, 2010 | https://doi.org/10.1371/journal.pntd.0000740
Farm-Level Risk Factors for Fish-Borne Zoonotic Trematode Infection in Integrated Small-Scale Fish Farms in Northern Vietnam
PLOS Neglected Tropical Diseases: published July 13, 2010 | https://doi.org/10.1371/journal.pntd.0000742
Kinetic Analysis of Ex Vivo Human Blood Infection by Leishmania
PLOS Neglected Tropical Diseases: published July 13, 2010 | https://doi.org/10.1371/journal.pntd.0000743
Integrated Surveys of Neglected Tropical Diseases in Southern Sudan: How Much Do They Cost and Can They Be Refined?
PLOS Neglected Tropical Diseases: published July 13, 2010 | https://doi.org/10.1371/journal.pntd.0000745
Family Relationship, Water Contact and Occurrence of Buruli Ulcer in Benin
PLOS Neglected Tropical Diseases: published July 13, 2010 | https://doi.org/10.1371/journal.pntd.0000746
Dengue Dynamics in Binh Thuan Province, Southern Vietnam: Periodicity, Synchronicity and Climate Variability
PLOS Neglected Tropical Diseases: published July 13, 2010 | https://doi.org/10.1371/journal.pntd.0000747
A Virulent Wolbachia Infection Decreases the Viability of the Dengue Vector Aedes aegypti during Periods of Embryonic Quiescence
PLOS Neglected Tropical Diseases: published July 13, 2010 | https://doi.org/10.1371/journal.pntd.0000748
Antiangiogenic and Antitumor Effects of Trypanosoma cruzi Calreticulin
PLOS Neglected Tropical Diseases: published July 6, 2010 | https://doi.org/10.1371/journal.pntd.0000730
Seasonal and Regional Dynamics of M. ulcerans Transmission in Environmental Context: Deciphering the Role of Water Bugs as Hosts and Vectors
PLOS Neglected Tropical Diseases: published July 6, 2010 | https://doi.org/10.1371/journal.pntd.0000731
Serological Studies of Neurologic Helminthic Infections in Rural Areas of Southwest Cameroon: Toxocariasis, Cysticercosis and Paragonimiasis
PLOS Neglected Tropical Diseases: published July 6, 2010 | https://doi.org/10.1371/journal.pntd.0000732
Specific Humoral Immunity versus Polyclonal B Cell Activation in Trypanosoma cruzi Infection of Susceptible and Resistant Mice
PLOS Neglected Tropical Diseases: published July 6, 2010 | https://doi.org/10.1371/journal.pntd.0000733
Where Do We Go from Here? Prevalence of Trachoma Three Years after Stopping Mass Distribution of Antibiotics in the Regions of Kayes and Koulikoro, Mali
PLOS Neglected Tropical Diseases: published July 6, 2010 | https://doi.org/10.1371/journal.pntd.0000734
Response to Treatment in a Prospective Cohort of Patients with Large Ulcerated Lesions Suspected to Be Buruli Ulcer (Mycobacterium ulcerans Disease)
PLOS Neglected Tropical Diseases: published July 6, 2010 | https://doi.org/10.1371/journal.pntd.0000736
Phase II Evaluation of Sensitivity and Specificity of PCR and NASBA Followed by Oligochromatography for Diagnosis of Human African Trypanosomiasis in Clinical Samples from D.R. Congo and Uganda
PLOS Neglected Tropical Diseases: published July 6, 2010 | https://doi.org/10.1371/journal.pntd.0000737
Comparison of Three Commercially Available Dengue NS1 Antigen Capture Assays for Acute Diagnosis of Dengue in Brazil
PLOS Neglected Tropical Diseases: published July 6, 2010 | https://doi.org/10.1371/journal.pntd.0000738