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Fig 1.

Overview of the proposed workflow.

(A) Multi-ACPNet architecture overview. First, ESM C features are extracted and fed into the (B) Sequence Multi-Scale Network to capture multi-scale sequence features. Then, graphs are constructed using trRosetta, with domain knowledge features and sequence-based learned features as node features. (C) The Graph Multi-Scale Network performs multi-scale structural feature learning on both the full graph and key subgraphs. The Classifier outputs the final prediction results.

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Fig 2.

ROC curves of the optimal model through 5-fold cross-validation on AntiCP 2.0 training datasets.

(A) Cross-validation result on the AntiCP 2.0_Main training dataset. (B) Cross-validation result on the AntiCP 2.0_Alternate training dataset.

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Table 1.

Performance comparison between the proposed method and existing methods on the ACP-Mixed-80 dataseta.

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Table 1 Expand

Table 2.

Performance comparison between the proposed method and existing methods on the AntiCP 2.0 independent test dataseta.

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Table 2 Expand

Table 3.

Performance comparison between the proposed method and existing methods for ACP functional activity predictiona.

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Fig 3.

Performance comparison between the proposed method and existing methods across seven cancer types.

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Fig 4.

Performance comparison of sequence-only and structure-only models on the AntiCP 2.0_Main test set.

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Fig 5.

t-SNE visualization of feature representations from the penultimate layer on AntiCP 2.0_Main test set.

(A) ACPNet_no_Graph feature distribution. (B) ACPNet_no_Seq feature distribution. (C) Multi-ACPNet feature distribution.

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Fig 6.

Agglomerative clustering of feature vectors from the penultimate network layer on ACP-Mixed-80 test set.

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Fig 7.

Comparative performance on the ACP toxicity test set ((s2).

(A) Confusion matrix for ToxGIN. (B) Confusion matrix for Multi-ACPNet.

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