Fig 1.
Workflow visualization of the application, illustrating the required steps and exportable outputs.
Steps include data upload or sample selection, and distance measure selection. Blue nodes represent available visualizations, each may be further configured and exported.
Fig 2.
Distinct behavior charts for subject 1 (top left and right) and subject 5 (bottom left and right) from the sample data of Neolamprologus multifasciatus from the website (S1 Dataset).
The total count for distinct behaviors is shown at the left, with the relative proportion per behavior on the right.
Fig 3.
Behavioral timelines for subject 9 from the sample data of Neolamprologus multifasciatus from the website (S1 Dataset).
The timeline for the behaviors is shown above. They describe the same data as Fig 2. The timeline for the corresponding behavioral categories is directly below.
Fig 4.
Temporal occurrences charts generated from sample data from the website showing the time on the x axis and the cumulative count of selected behaviors per individual on the y axis.
Data for the two shown plots originate from two different recordings: Neolamprologus multifasciatus (S1 Dataset) on the left and Lamprologus ocellatus (S2 Dataset) on the right side, each documenting behavior over a 20-minute period. There are 7 individuals recorded on the left and 10 on the right.
Fig 5.
Interaction networks generated from the sample data of Lamprologus ocellatus from the website (S2 Dataset).
On the left side, all interactions i. e. behavior emanating from one individual and directed towards another are displayed, the betweenness of nodes (individuals) is mapped to node size and color brightness. On the right side detailed views for behavior emanating from subject 1 (top) and targeting subject 9 (bottom) are depicted.
Fig 6.
Behavior transition networks generated from the sample data of Neolamprologus multifasciatus from the website (S1 Dataset).
Both are set to use behavioral categories as nodes. On the left hand side, the average time a behavioral category is shown is mapped to node label, size and color brightness, while on the right side the total time is applied as mapping. The node properties are set as linear normalized. Edge values depict the transitional frequency from one category to another, rounded to the second digit. The edge width is set to be dependent on the edge weighting.
Fig 7.
Distance matrix from pairwise comparisons of behavior sequences of the two cichlid species Neolamprologus multifasciatus and Lamprologus ocellatus (S4–S17 Datasets), using the Network Portrait Divergence as distance measure.
As distances are symmetric and a network has a distance of zero to itself, the distance matrix is itself symmetric with zeros on the diagonal.
Fig 8.
Two-dimensional embeddings of distances between transition networks as result of multi-dimensional scaling (MDS) applied to the distance matrix (Fig 7), where distances between single data points are preserved as good as possible while reducing dimensions.
Data of two cichlid species, Neolamprologus multifasciatus and Lamprologus ocellatus (S4–S17 Datasets), are compared pairwise with the Network Portrait Divergence applied as distance measure. The indices correspond to the first letters of the species, where the appended number allows the assignment to the same record in the dendrogram in Fig 9. The four embeddings were created with the parameter n_init set to 400 (the number of times the algorithm runs) and with the starting states 0, 40, 65 and 77 from the top left to the bottom right corner.
Fig 9.
Dendrograms of distances across transition networks as a result of agglomerative clustering of the distance matrix (Fig 7).
The average and complete distances between clusters are set as linkage criteria, on the left and on the right, respectively. Merged clusters with distances below 0.4 are set to have distinct colors. Data of two cichlid species, Neolamprologus multifasciatus and Lamprologus ocellatus (S4–S17 Datasets), are compared pairwise and the Network Portrait Divergence is applied as distance measure. The indices correspond to the first letters of the species, where the appended number allows the assignment to the same record in the plot in Fig 8.