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Fig 1.

Overview of components of an ISCT (necessary components in white boxes, possible components in light blue boxes).

Left side: a virtual cohort (set of virtual patients) can be created given a baseline patient model and a cohort generation method. Coupling with a device model, and potentially other sub-models, results in the virtual cohort for the ISCT, which is run using the ISCT study protocol to obtain the ISCT results. The right-hand box shows four approaches to generating a virtual cohort (not comprehensive). Approach A): Construct subject-specific virtual patients from subject-specific data. B) Construct subject-specific virtual patients and alter to create synthetic patients. C) Generate synthetic patients by sampling from probability distributions derived from subject-specific virtual patients; D) Generate synthetic patients by sampling from prescribed probability distributions. Two parameters, p1 and p2, shown for simplicity. See text for discussion.

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Table 1.

Overview and comparison of ISCTs discussed in Section 2.3.

MR: magnetic resonance, RF: radiofrequency, AUC: area under receiver operator characteristic curve, ODE: ordinary differential equation, CGM: continuous glucose monitor, FEA: finite element analysis.

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Table 2.

Selection of possible validation tests that could be performed to evaluate an ISCT, identified in Section 3.2.

This is not intended to be a comprehensive list of options; many options or variants are possible.

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Fig 2.

Possibilities for validation of device and patient models (not comprehensive).

Equality sign with question mark indicates "Compared as part of validation activity”. See text for discussion.

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Fig 3.

Possibilities for cohort validation (not comprehensive).

Equality sign with question mark indicates "Compared as part of validation activity”. (a) N subject-specific virtual patients each individually validated against data from the corresponding real-world subjects. (b) Distribution of a model output across the virtual cohort validated against corresponding data from the real-world population. (c) Results of an ISCT directly compared to a real-world clinical trial.

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Fig 4.

Overview of sub-steps in Step 4 of the workflow.

Equality sign with question mark indicates "Compared as part of validation activity”.

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Table 3.

Credibility factors and gradations that could be used for population-level validation of the virtual cohort.

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