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Fig 1.

The evolution of a graphical abstract, from sketch (A) to a rapid Biorender draft (B) and final implementation in a graphical software program (C). All drawings by HKJ, licensed under CC0, https://doi.org/10.6084/m9.figshare.24486061.v1.

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Fig 2.

(A) All pictograms used have similar overall appearance (color, size, design, modified from [11]). (B) Poor combination of pictogram for the same workflow: pictograms have different overall appearance. Icons in A: Fontawesome, Fonticons, Inc. Icons in B: Microscope: Bioicons DBCLS https://togotv.dbcls.jp/en/pics.html is licensed under CC-BY 4.0; Laptop: Icon by Simon Dürr https://twitter.com/simonduerr is licensed under CC0 https://creativecommons.org/publicdomain/zero/1.0/; Image/slider: see A; Colors and people: drawn by HKJ; Newspaper: https://www.svgrepo.com/svg/301104/newspaper-news, CC0.

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Fig 3.

Common chart types with few data categories/points each that convey high level information.

Note that the core message (increases, is most, one third…) is communicated without axis details, labels, and legends.

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Fig 4.

Different dimensions (A) and how they merge with text on a page (B).

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Fig 5.

Different layouts for graphical abstracts with clear start and end.

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Fig 6.

Common arrow types (A) and arrows in context (B).

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Fig 7.

Color can highlight (A), encode numbers (B), or show natural appearance (C) in graphical abstracts. Be careful with your color choice when using a colored background. Image: Albrecht Dürer, Public domain, via Wikimedia Commons (https://commons.wikimedia.org/wiki/File:Albrecht_D%C3%BCrer_-_Hare,_1502_-_Google_Art_Project.jpg).

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