Fig 1.
A cross sectional view of the petridish with the fish skin sample.
Fig 2.
(a) A simplified PAM experimental setup. OI: Optical isolator, BS: Beam splitter, ND filter: Neutral density filter, PD: Photodiode, OL: Objective lens. TL: Tube lens. (b) The schematic view of the PAM setup (c) A simplified experimental setup for SAM.
Fig 3.
(a)line profile of a sharp object fitted with error function (in red) (b) Photoacoustic image of the edge (c) Photoacoustic image showing the boundary.
Fig 4.
(a)C-scan for PAM (b)B-scan for PAM, Hot colormap is chosen for PAM representation (c) C-scan for SAM (d) B-scan for SAM, gray colormap is chosen for SAM representation. The yellow line in B-scan indicates the region for which C-scan has been shown. The size of the C-scan images is 4mm*3mm and the pixel size is 10 μm.
Fig 5.
(a) side view of SAM & PAM overlayed image from dataset1 having dimensions (4mm*3mm) (b) angular view representation of the overlayed image(c) The SAM signal where the white shadowed part shows the signal responsible for the image. (d) The PAM signal where the white shadowed part shows the signal responsible for the image and the amplitude (Arbitrary units) (e) Volume histogram of the signal where the bins are from 0 to 256.
Fig 6.
(a) side view of SAM & PAM overlayed image from dataset2 having dimensions (4mm*3mm) (b) angular view representation of the overlayed image (c) The SAM signal where the white shadowed part shows the signal responsible for the image (d) The PAM signal where the white shadowed part shows the signal responsible for the image and the amplitude is in Arbitrary units(d) Volume histogram of the signal.
Fig 7.
(a) side view of SAM & PAM overlayed image from dataset3 which has scattered pigments having dimensions (4mm*3mm) (b) angular view representation of the overlayed image.
Fig 8.
(a) PAM image(4mm*3mm) showing the optical absorption map. (b) A rectangle showing the area taken for three-dimensional voxel representation in (c) The optical image of the same region (The optical image was taken with 4X lens for high field of view and better overlapping).
Fig 9.
Histogram for calculation of voxels which contribute to the signal (a)PAM (b) SAM signal. The unit of voltage is in Volts.