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Fig 1.

Simplified overview of the MUFFIN workflow.

All three steps (Assemble, Classify, Annotate) from top to bottom are shown. The RNA-Seq data for Step 3 (Annotate) is optional. Differential reads are other read data sets that are solely used for "differential coverage binning" to improve the overall binning performance.

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Fig 2.

Example snippets of the sub-workflow results of step 3 (Annotate).

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Fig 3.

Quality of meta-assembled genomes (MAGs).

[A] Quality overview of 1122 MAGs by plotting size to completeness and coloring based on contamination level. [B] N50 comparison between each bin of five selected samples from the Bioproject PRJEB34573 before and after individual bin reassembly.

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