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Fig 1.

Image with ground truth segmentation.

Nucleus signal (a), cytoplasm signal (b), and ground truth segmentation (c).

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Fig 2.

Feature pyramid fusion of nucleus features.

Pre-trained nucleus features (violet) are fused with features of the feature pyramid in the cell detection and segmentation model (green) by either concatenation or addition.

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Table 1.

Reduced ResNet-50 architecture.

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Fig 3.

Including nucleus information for cell segmentation.

(a) without nucleus information, (b) with additional input for the nucleus channel, and (c) with fused nucleus features.

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Fig 4.

Weights for instances.

For each box proposal, crops are resized to 28 × 28 pixels. Crop from the input image (a), full-image segmentation mask (b), cell mask (c) and weight matrix (d).

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Fig 5.

Visualization of cell segmentation errors on a 256 × 256 patch of clustered cells in test data: Predicted masks (red) differ only slightly from ground truth masks (white).

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Table 2.

Detection and segmentation results for the nuclei test dataset in terms of AP.

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Table 3.

Detection results for the cells test dataset in terms of AP.

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Table 4.

Segmentation results for the cells test dataset in terms of AP.

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Table 5.

Detailed cell segmentation results for an IoU threshold of 0.75 on the cells test dataset.

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Table 6.

Detection results for clustered cells test dataset in terms of AP.

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Table 7.

Segmentation results for clustered cells test dataset in terms of AP.

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Fig 6.

APs for cell segmentation on clustered cells.

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Fig 7.

Visualization of segmentation of clustered cells.

Top: nucleus signal (a), cytoplasm signal (b), and ground truth segmentation (c). Bottom: Instance segmentations predicted by for model without nucleus information (d), with nucleus channel (e), and FPF ⊕ with weighted loss (f).

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Fig 8.

Cell segmentation of clustered cells by Feature Pyramid Fusion (FPF) on a 512 × 512 patch.

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