Fig 1.
Cells with CD30 immunostaining are colored red. There is one trinucleated HRS cell in the left part and one binucleated HRS cell in the middle upper part. The cell nuclei colored blue are stained using hematoxylin.
Fig 2.
The fractions of cells classified according to the PC = 0 to PC = 7 averaged over the 35 images.
Fig 3.
The fractions of small and large cells.
The fractions of small (PC = 0, 2, 4, 6) and large (PC = 1, 3, 5, 7) PC as function of the diagnosis. The error bars show the standard deviation. The fraction of large PC in images of NScHL, MCcHL, and LA were statistically indistinguishable.
Fig 4.
The diameter distribution of cells.
The bar chart shows the distribution of the diameters for all diagnoses.
Fig 5.
The corrected diameter distribution of cells.
The bars show the relative fractions of HRS cells for the diagnoses NScHL and MCcHL, respectively. The relative fractions were corrected for the background of activated lymphocytes, see text.
Fig 6.
Neighborhood relations for all diagnoses.
Each node represents one of the eight PC. Arrows are drawn between nodes if the absolute value of the score of neighborhood relation exceeds 50% of the maximally possible value. Green and red arrows represent favored and unfavored neighborhood relations, respectively. The thickness of the arrows correlates with the absolute value of the score. Thick green and red arrows stand for large positive and large negative scores, respectively.
Fig 7.
Disease-specific neighborhood relations.
Networks of neighborhood relations A) in NScHL, B) in MCcHL and C) in LA. Each node represents one of the eight PC. The thickness of the arrows correlates with the absolute value of the score. Green and red arrows represent favored and unfavored neighborhood relations, respectively.
Table 1.
Mean distances of PC to the nearest neighbor in images of all diagnoses.
Fig 8.
Neighborhood of small, round cells in a low density area.
An exemplary subsection of an histological image with diagnosis MCcHL (ID 5722). The green points depict small, round cells (PC = 0) with a nearest neighbor of the same PC. The red points indicate all remaining PC. The clusters of small, round cells seem to be more often located in areas of low cell density (black arrows), whereas cell clusters of other PC seem to be more often in areas of higher cell density (white arrows).
Fig 9.
The eight profile classes numbered from 0 to 7 and their morphological descriptions.
Fig 10.
Exemplary CD30-positive cells for the eight morphologic profile classes (PC). The tissue sections display A) for PC 0 a small, round cell, B) for PC 1 a large round cell, C) for PC 2 a small, elongated cell, D) for PC 3 a large, elongated cell, E) for PC 4 a small, frayed cell, F) for PC 5 a large, frayed cell, G) for PC 6 a small, elongated, frayed cell, and H) for PC 7 a large, elongated, frayed cell.
Fig 11.
A) depicts a subsection of a schematic histological image that contains ten cells, four cells of PC = 0, two cells of PC = 1, and each single cell of PC = 3, 4, 5, 7, respectively. B) gives the neighborhood table that contains for each cell the PC and the PC of the nearest neighbor called neighbor profile class (NPC).
Table 2.
Significance matrix for the image ID = 1721 of the diagnosis MCcHL.