Fig 1.
Fit of model to IAV infection data at five different inoculum doses.
Kinetics for 6 of the seven model compartments for the best fit model are plotted. Since infected cells kinetics very closely follows virus kinetics, we did not plot it. Dashed horizontal line indicates the limit of detection for virus load. Best fit parameter values are provided in S1 Text. Data was available for virus load and cell damage. Virus load data was reported as hemagglutination units and cell damage was reported as percent lung cells that are pathological. Model virus load is in the same units as the experimental data. All other model quantities are in units of numbers. Damage in the model is measured as number of dead cells. Both dead cells and antibodies are rescaled as described in the method section to allow comparison between model and data. The plot shows the rescaled quantities.
Fig 2.
Fit of model to HPIV infection data at five different inoculum doses.
Kinetics for 6 of the seven model compartments for the best fit model are plotted. Since infected cells kinetics very closely follows virus kinetics, we did not plot it. Dashed horizontal line indicates the limit of detection for virus load. Best fit parameter values are provided in S1 Text. Data was available for virus load and some antibody levels. Virus load data was reported as plaque forming units per gram of lung and antibody titer was reported as plaque reduction neutralization units. Model virus load is in the same units as the experimental data. All other model quantities are in units of numbers. Damage in the model is measured as number of dead cells. Both dead cells and antibodies are rescaled as described in the method section to allow comparison between model and data. The plot shows the rescaled quantities.
Fig 3.
Protection as function of antibody levels (k1 = 1, k2 = log(100)).
Protection is the fraction of individuals in a population who are protected by the vaccine for a given level of antibodies. Antibody levels are on an arbitrary unit.
Fig 4.
Data and best fit model for the connection between immune response and symptoms.
The innate response is the scaled sum of IFN-a, IL6, IL8, and TNF-a, symptoms are the total symptom score, both quantities are from [28]. The line shows the best fit provided by the equation mapping innate response to symptoms/morbidity.
Fig 5.
IAV model simulation for a range of inoculum doses.
All plot settings are the same as described for Fig 1.
Fig 6.
HPIV model simulation for a range of inoculum doses.
All plot settings are the same as described for Fig 2.
Fig 7.
Inoculum dependent protection and damage for the IAV infection model.
Protection was determined based on antibody levels predicted by the model and computed using the equation for protection, P(A), described above. Morbidity was determined from the innate response levels predicted by the model and computed using the MAUC equation described above. To allow for better comparison with protection, we also scaled the MAUC values by their maximum.
Fig 8.
Inoculum dependent protection and damage for the HPIV infection model.
Protection was determined based on antibody levels predicted by the model and computed using the equation for protection, P(A), described above. Morbidity was determined from the innate response levels predicted by the model and computed using the MAUC equation described above. To allow for better comparison with protection, we also scaled the MAUC values by their maximum.
Fig 9.
Model for non-replicating vaccine.
Model parameters were set to dV = 0.1, , hV = 1E5, gF = 1E3, Fmax = 1E3, hF = 100, gB = 0.1, rA = 1, dA = 1E − 6, kA = 1E − 6. Initial conditions are F = 1, B = 1, A = 0 and varying values for antigen load.
Fig 10.
Inoculum dependent protection and damage for the inactivated vaccine model.