Fig 1.
Comparison of sashimi plots generated by ggsashimi and IGV.
Sashimi plots of 12 ENCODE samples belonging to 3 cell type groups (endothelial, epithelial and mesenchymal) for the region chr10:27040584-27048100 obtained by ggsashimi (A) and the sashimi-plot utility within IGV (B). The inclusion level of the exon chr10:27044584-27044670 is clearly higher in mesenchymal cells (blue), followed by epithelial (green) and endothelial cells (red). While this trend is barely observable in the IGV sashimi, which becomes complex and confusing with multiple samples, as it makes one sashimi plot per sample; the --overlay option of ggsashimi allows aggregating samples belonging to the same groups, providing a much better overview of the event. In addition, the presence of long introns flanking the exon of interest substantially enlarges the connective elements and hinders visualization in IGV sashimi. Conversely, ggsashimi avoids this problem thanks to its --shrink option, which updates the original intron lengths, enhancing visualization.