Fig 1.
Condition-dependent variants of the NR2 subunits of human NMDA receptors.
(A) The expression profiles of GRIN2A and GRIN2B, the two genes encoding NR2A and NR2B in human cerebellum (CBC). The expression level of GRIN2B (NR2B) declines during prenatal and early childhood, while the expression level of GRIN2A (NR2A) rises during that period. (B) Same as A for RNA-seq Brainspan data [34]. (C) Distribution of correlation values across various gene pairs ad regions in the data of [32]. The curve marked ‘pathways’ corresponds to the all pairs from the brain-related keg pathways. Curves marked with percentages correspond to sets of gene pairs whose sequence overlap is in the designated range, based on biomart overlap measure. Asterisks mark bins of the histogram where the observed number pairs in the 17 pathways is significantly larger (permutation test, p <10–3) from that obtain at random. Dashed line shows the fraction expected at random. (D) Same as C for RNA-seq Brainspan data [34].
Fig 2.
Dissimilarity significance of the top 20 genes in 17 brain regions measured by Kang et al. [32] and in the prefrontal cortex measured by Colantouni et al. [33].
Colors correspond to negative log10(p-values) of Pearson correlation. Gray pixels denote regions in which the range of expression levels (maximum-minimum) was below 1.5 for at least one gene, or an insignificant q-value. The bottom row shows dissimilarity for an interesting pair of serotonin receptors. Brain regions are grouped and sorted as follows: prefrontal: DFC, OFC, VFC, MFC; frontal-parietal: M1C, S1C, IPC; temporal-occipital: ITC, STC, A1C, V1C; subcortical: AMY, CBC, HIP, MD, STR; prefrontal data from [33]: PFC. Region codes are listed in the Methods section. The genes coding for histone deacetylase, HDAC2 and HDAC11 exhibit significant dissimilarity levels in all brain areas. The genes coding for Neurotensin receptor NTSR1 and NTSR2 exhibit significant dissimilarity mainly in cortical regions and the striatum.
Fig 3.
(A) An example of fitting a cubic spline[80] to the expression of HTR1A and HTR5A in the primary visual cortex (V1C). Circles denote measured data points and solid lines denote a cubic spline fit. (B) Expression levels of HTR1A and HTR5A. Circles denote the expression of prenatal subjects, triangles of postnatal. The color scale denotes the age of each donor, with older subjects in light yellow and young subjects in dark. The expression is anti-correlated (ρ = -0.58). (C) After removing the population effect of age, the expression of HTR1A and HTR5A is positively correlated across subjects (ρ = 0.53, q-value < 10−4, Pearson). (D) The magnitude of age-corrected correlation (residuals) of 20 CDV pairs, ranked by the mean correlation over regions.
Fig 4.
A novel CDV pair in the glycine receptor subunits GLRA2 and GLRA3.
(A) A diagram of glycine receptor components (B) The gene expression profiles of glycine receptor subunits as measured by Kang et al. (2011) in the human posterior inferior parietal cortex (IPC). (C) The gene expression profiles of glycine receptor subunits as measured by Kang et al. (2011) in the human cerebellar cortex. (D) Subject to subject age corrected correlation within two age groups, prenatal (10PCW-birth), and postnatal (0-30Y).
Fig 5.
A novel CDV pair of serotonin-receptor genes HTR1A and HTR5A.
(A) A diagram of the serotonergic synapse. (B) The gene expression profiles of HTR1A and HTR5A serotonin receptors as measured by [32] in four regions of the human prefrontal cortex: dorsolateral, medial, orbital and ventrolateral prefrontal cortex. (C) The gene expression profiles of HTR1A and HTR5A serotonin receptors as measured by [33] in the prefrontal cortex. (D) Subject to subject correlation, after removing the population effect of age, between the expression levels of HTR1A and HTR5A within two age groups, prenatal (10PCW-birth), and postnatal (0-30Y) in the hippocampus.
Fig 6.
Timing and spatial differences of the NR2A/2B developmental switch.
In human cortex (RNA-seq:A, microarrays: D) and Hippocampus (RNA-seq:B; microarrays:E) childhood expression level of NR2B exhibit little significant change, and NR2A may be rising slowly. In the cerebellum (RNA-seq: C; microarrays: F), a more significant switch is observed. In all cases, the largest changes in expression levels occur before birth, and much smaller changes are observed postnatally.
Table 1.
Donors age groups following [32].
Table 2.
List of KEGG brain-related pathways used to search for potential CDVs.