Fig 1.
Why correlations between relative abundances tell us absolutely nothing.
These plots show two hypothetical mRNAs that are part of a larger total. (a) Seven pairs of relative abundances (mRNA1/total, mRNA2/total) are shown in red, representing the two mRNAs in seven different experimental conditions. The dotted reference line shows (mRNA1 + mRNA2)/total = 1.) Rays from origin through the red points show absolute abundances that could have given rise to these relative abundances, e.g., the blue, green or purple sets of points (whose Pearson correlations are −1, +1 and 0.0 respectively). (b) Relative abundances that are proportional must come from equivalent absolute abundances. Here the blue, green or purple sets of point pairs have the same proportionality as the pairs of relative abundances in red, though not necessarily the same order or dispersion.
Fig 2.
Fission yeast gene expression data of Marguerat et al. (a) Absolute and (b) relative abundances of 3031 yeast mRNAs over a 16-point time course.
y-axes are scaled logarithmically; x-axes are on a square-root scale for clarity. Each grey line represents the expression levels of a particular mRNA. The red and blue pairs of mRNAs are discussed later in this paper.
Fig 3.
Correlations between relative abundances bear no relationship to the corresponding correlations between absolute abundances.
(a) The pair of mRNAs labeled in red in Fig. 2, shown on a linear scale. Values have been scaled and translated to have zero mean and unit variance. Upper panels show absolute abundances; the lower show relative abundances. The left panels show mRNA values over time; the right show the value of one mRNA plotted against the other at each time point. The correlation between the relative abundances is almost the complete opposite of that between the absolute abundances of this pair of mRNAs. (b) 2D histogram of the sample correlation coefficient observed for the relative abundances of a given pair of mRNAs, against the correlation observed for the absolute abundances of that same pair, over all pairs. The red and blue points correspond to the red and blue pairs of mRNA in Fig. 2. White contour lines are shown at intervals of 100 counts. The top marginal histogram shows that the absolute abundances of most pairs are very strongly correlated. The right marginal histogram shows “the negative bias difficulty” [4].