Figure 1.
The flowchart of bioimage informatics for drug and target discovery.
Figure 2.
A representative image of Drosophila Kc167 cells treated with RNAi.
The red, green, and blue colors are the DNA, F-actin, and α-tubulin channels.
Figure 3.
Examples of HeLa cell nuclei and cell cycle phase images.
(A) A frame of HeLa cell nuclei time-lapse image sequence; (B) Example images of four cell cycle phases.
Figure 4.
A representative 2D neuron images.
The bright spots near the backbones of neurons are the dendritic spines.
Figure 5.
A representative image of neurites.
Red indicates nuclei and green represents neurites.
Figure 6.
An example of blob-structure (HeLa cell nuclei) detection.
The red spots indicate the detected centers of objects.
Figure 7.
A representative neurite image for centerline detection.
Figure 8.
An example of neurite centerline detection.
(A) The centerline confidence image obtained by using the local Gaussian derivative features. Higher intensity indicates higher confidence of pixels on the centerlines. (B) The neurite centerline detection result image. Different colors indicate the disconnected branches.
Figure 9.
An example of HeLa nuclei segmentation using the seeded watershed algorithm.
The green contours are the boundaries of nuclei.
Figure 10.
An example of segmentation of Drosophila cell images using the level set approach.
Figure 11.
Time-lapse images indicating cell cycle progression.
The cell in the red square in the first frame (A) divided into two cells in frame 60 (B). The descendent cells divided again in frame 152 and 156 respectively as shown in the red squares in (C) and (D).
Figure 12.
Examples of cell migration trajectories.
Different colors represent different trajectories.
Figure 13.
Examples of cell lineages constructed by the tracking algorithm.
The black numbers are the time of cell division (hours). The bottom red numbers indicate the number of traces, and the numbers inside circles are the labels of cells in that frame.
Figure 14.
A segment of cell cycle procession sequence.
Four cell cycle phases, interphase, prophase, metaphase, and anaphase, appear in order.
Figure 15.
The graphical representation of cell cycle phase identification.
Figure 16.
A representative image of Drosophila cells with three phenotypes: (A) Normal, (B) Ruffling and (C) Spiky phenotypes.
Figure 17.
An illustration of drug profiling using the normal vector of hyperplane of SVM.
The red and blue spots indicate the spatial distribution of cells in the numeric feature space. The yellow arrow represents the normal vector of the hyperplane (the blue plane). The top left and bottom right (MB231 cell) images are from drug treated and control conditions respectively.
Table 1.
List of publicly available bioimage informatics software packages.