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Table 1.

MK table.

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Table 2.

SnIPRE coefficients and population genetic parameters.

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Figure 1.

Example joint distribution of the estimated selection effect and the constraint effect for a particular gene.

Data simulated using PRFREQ. The blue asterisk denotes the true location of parameters.

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Table 3.

SnIPRE predicted mutation counts.

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Table 4.

Expected mutation counts.

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Table 5.

False positive rate.

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Table 6.

False positive rate and demography.

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Figure 2.

Classification of constraint.

Top: Distribution 1, 2, and 3 of used in the coalescent simulations for Table 7. Bottom: Proportion of constraint effects classified as significant by SnIPRE; x-axis is true proportion of non-lethal mutations, .

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Table 7.

Realized coverage of 95% CI for and γ when , varies, and there is linkage among sites.

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Figure 3.

Comparison of estimates of constraint when (no constraint).

A: The distribution of constraint estimates. B: Constraint estimates versus the selection strength.

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Figure 4.

Classification of selection effect for Drosophila-like simulations.

Shaded regions of histogram represent the proportion of genes under selection classified as under selection; x-axis is true selection coefficient; .

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Figure 5.

Classification of selection effect for human-like simulations.

Shaded regions of histogram represent the proportion of genes under selection classified as under selection; x-axis is true selection coefficient; .

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Table 8.

Selection classification for simulations by method.

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Figure 6.

True positive rate versus false discover rate.

Results for data set of 2,000 genes, 550 of the genes are under selection with or .

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Figure 7.

Distribution of residuals for selection coefficient estimates by method.

The top row displays the distribution of constraint, the middle row displays residuals for simulations using ; the bottom row displays residuals for simulations using . Residuals grouped by true selection strength.

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Figure 8.

D. simulans estimated selection effects and non-synonymous effects for 8,887 genes.

Plots A and B shows the estimated selection effects using SnIPRE and B SnIPRE respectively.

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Figure 9.

Human estimated selection effects and non-synonymous effects for 11,624 genes.

Plots A and B shows the estimated selection effects using SnIPRE and B SnIPRE respectively. B SnIPRE classifies far more genes as having a negative average selection effect, and this difference can be explained in part by the construction of 95% confidence interval versus the credible interval.

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