Figure 1.
Comparing skew and mean replication timing (MRT).
(A) profile along a 11.4 Mb long fragment of human chromosome 10 that contains 6 skew N-domains (horizontal black bars) bordered by 7 putative replication origins
to
. Each dot corresponds to the skew calculated for a window of 1 kb of repeat-masked sequence. The colors correspond to intergenic (black),
genes (red) and
genes (blue). (B) Corresponding cumulative skew profile
obtained by cumulative addition of
-values along the sequence. (C) MRT profiles from early, 0 to late, 1 for BG02 (green), K562 (red) and GM06990 (blue) cell lines. (D) Correlations between
and
, in BG02 (100 kb windows) along the 22 human autosomes; colors as in (A); the corresponding Pearson correlations are given in Table 1. (E) Average
profiles (
SEM) in the 663 skew N-domains after rescaling their length L to unity; colors as in (C).
Table 1.
Compostional skew and derivative of the replication timing profile correlate.
Figure 2.
Replication timing U-domains in different human cell lines.
(A) Average MRT profiles ( SEM) inside detected replication U-domains (Table 2). (B) Corresponding average
profiles (
SEM). In (A) and (B), each cell line is identified by a color: BG02 (green), K562 (red), GM06990 (blue), BJ R2 (magenta), and HeLa R2 (cyan). (C) The 2534 BG02 U-domains were centered and ordered vertically from the smallest (top) to the longest (bottom). The MRT profile of each domain is figured along a horizontal line using the MRT (BG02) color map. (D) Same as in (C) but for
using the
(BG02) color map.
Table 2.
Replication domains characteristics.
Table 3.
Correspondence between replication domains.
Figure 3.
Analysis of chromatin marks along U-domains.
Over representation of open chromatin markers (Material and Methods) at replication timing U-domain borders relative to the corresponding genome-wide value. (A) Mean coverage by DNase I hypersensitive zones, as a function of the distance to the closest U-domain border in BG02 using DNase H1-hESC data (green, genome-wide mean value = 0.0073), K562 using DNase K562 data (red, genome-wide mean value = 0.0138), GM06990 using DNase GM06990 data (blue, genome-wide mean value = 0.0107). (B) Proportion of clones presenting a ratio of “open” over input chromatin greater than 1.5 versus the distance to the closest U-domain border in GM06990 for four U-domain size categories: L0.8 Mb, 0.8 Mb
L
1.2 Mb, 1.2 Mb
L
1.8 Mb and 1.8 Mb
L
3 Mb from light to dark blue curves (genome-wide mean value = 0.20). (C) Mean coverage by 1 kb-enlarged CpG islands as a function of the distance to the closest U-domain border in BG02 for the four U-domain size categories defined in (B) from light to dark green curves (genome-wide mean value = 0.0254). (D) Mean coverage by Pol II peaks as a function of the distance to the closest U-domain border in BG02 (green: Pol II in H1 ESC, genome-wide mean value = 0.0026), K562 (red: Pol II in K562, genome-wide mean value = 0.0024), GM06990 (blue: Pol II in GM12878, genome-wide mean value = 0.0097).
Figure 4.
Chromatin conformation data and U-domain compartmentalization of the genome.
(A) Hi-C proximity matrix corresponding to intrachromosome interactions on the 11.4 Mb long fragment of human chromosome 10 (Fig. 1), as measured in the K562 cell line (Material and Methods). Each pixel represents all interactions between a 100 kb locus and another 100 kb locus; intensity corresponding to the total number of reads is color coded according to the colormap (right). The dashed squares correspond to replication timing U-domains detected in the K562 cell line. (B) Number of interactions between two 100 kb loci versus the distance separating them (logarithmic scales) as computed genome wide (black) or in K562 replication U-domains only, for four U-domain size categories: L0.8 Mb, 0.8 Mb
L
1.2 Mb, 1.2 Mb
L
1.8 Mb and 1.8 Mb
L
3 Mb (from light to dark red). (C) Ratio of the number of interactions between two 100 kb loci inside the same U-domain at equal distance from its center and the number of interactions between loci on opposite sides and equal distance from a U-domain border, versus the distance between them; colors as in (B).
Figure 5.
Enrichment in insulator-binding protein CTCF at replication U-domains borders.
(A) Mean coverage by CTCF enriched signals versus the distance to the closest U-domain border in K562 cell line for four U-domain size categories: L0.8 Mb, 0.8 Mb
L
1.2 Mb, 1.2 Mb
L
1.8 Mb and 1.8 Mb
L
3 Mb, from light to dark red curves (genome-wide mean value = 0.0051). (B) Same as in (A) but for the GM06990 cell line (blue code shades) (genome-wide mean value = 0.0046).
Figure 6.
Modeling the spatio-temporal replication program.
Replication timing (A) and fork orientation
(B) of the configuration
where
corresponds to the origin
positioned at location
and firing at time
. Fork coming from
meets the fork coming from
at the space-time point
defined in Equation (11). The replication timing and fork orientation at the spatial position
are given by Equation (12) from which we deduce the relationship
and in turn Equation (14) for the replication fork polarity and the derivative of the MRT. In this picture of the spatio-temporal replication program, the replication fork velocity
is assumed to be constant and replication is bidirectional from each origin.