Table 1.
List of proteins ordered by functional category and changing when growth rate increases from 0.09 to 0.24 and 0.47 h−1 during continuous culture of L. lactis.
Figure 1.
Distributions of protein and mRNA data for different growth rates in L. lactis.
Protein concentrations (A), mRNA concentrations (B), mRNA/protein ratios (C) and their ratios between two different growth conditions (D) ranked in increasing order.
Figure 2.
Modeling of the cellular process.
Translation, dilution and degradation rates expressed respectively by k′[mRNA]), μ[protein] and k″[protein] where k′ is the translation efficiency, μ the growth rate and k″ the degradation rate constant.
Table 2.
Mean sum of squared residuals associated to different solutions to solve equation (2).
Table 3.
Modeling of the mRNA/protein ratios by a linear relation of μ2/α+ β/α.
Figure 3.
Distribution of translation efficiency and degradation rate constant for different growth rates in L. lactis.
Histograms for translation efficiency, k′, (A) and protein degradation rate, k″, (B) with coloured bars (black for μ = 0.88 h−1, dark grey for μ = 0.47 h−1, grey for μ = 0.24 h−1 and white for μ = 0.09 h−1) and lines indicating the Gaussian tendency curves.
Table 4.
Correlation analysis between amino-acid usage in L. lactis proteins and translation efficiencies or degradation rates.
Figure 4.
Competition between dilution and degradation rates for protein control.
Comparison of growth rate (μ: straight line) and median value of the degradation constant (k″: dotted line).
Table 5.
Protein control analysis in the different ranges of growth rate based on ρt calculation.