Figure 1.
Steps in the Derivation of the Nucleosome Scores S℘N℘ and S℘D℘N℘
(1) Obtain the sets of bound and unbound sequences ({X1,X2,…,Xn) and {Y1,Y2,…,Ym}, respectively) from a ChIP-chip experiment for a particular TF ((inidcated by a diamond).
(2) Determine the nucleosome occupancy O℘ for all the bound and the unbound sequences.
(3A) Compute the simple nucleosome score S℘N℘(Xi, j) for each W-mer starting at position j in the bound sequence Xi by averaging the accessibility (1 − O℘) over all positions in the W-mer.
(3B) Compute the discriminative nucleosome score S℘D℘N℘(Xi, j) for each W-mer starting at position j in sequence Xi, using the accessibility (1 − O℘) over all occurrences of this W-mer in both the bound and the unbound sequences (see Materials and Methods for details). All the sequences and scores depicted in this figure correspond to the TF Reb1 profiled in YPD and use occupancy predictions from the computational model of Segal et al. The black boxes on the bound DNA sequences indicate matches to the Reb1 motif.
Figure 2.
Performance of the Three Positional Priors
A dark orange (light grey) square in each column indicates the situation where the respective prior succeeds (fails) in finding the true motif. There are 23 = 8 possible combinations of successes or failures for the three priors. These are represented by the eight columns, which are ordered based on the success or failure of PRIORITY-D℘N℘. The number of sequence-sets (out of the total 156 sequence-sets) falling into each category is indicated below the respective column.
Figure 3.
Distribution of S℘N℘, S℘D℘N℘, and S℘D℘N℘ ′ in the Sequence-Set for Leu3 Profiled in YPD
The distribution of scores over each unique 10-mer occurring in the Leu3_YPD sequence-set shown as a percentile plot (on the left) and as a histogram (on the right) computed according to: (A) S℘N℘ (averaged over each 10-mer) using predictions from computational model of Segal et al., (B) S℘D℘N℘ using predictions from computational model of Segal et al., and (C) S℘D℘N℘ ′ using low-resolution nucleosome data from Lee et al. The three colored dots marked on each figure indicate the positions of the only three 10-mers matching the Leu3 motif CCGGNNCCGG present in Leu3_YPD. The red dot corresponds to CCGGTACCGG (see text). The mass to the right of the dots in each graph reveals the fraction of 10-mers scoring higher.
Figure 4.
Nucleosome Occupancy and the Values of S℘D℘N℘ over Four Intergenic Sequences
(A) iYDR190C in Cbf1_SM, (B) iYAR007C in Mbp1_H2O2Hi, (C) YJLWdelta16 in Gcr1_YPD, and (D) iYBR043C in Gcn4_YPD. The boxes indicate binding sites annotated by Harbison et al. [2]. S℘D℘N℘ at the locations of each of these binding sites has a high value relative to the rest of the sequence regardless of the S℘N℘ score at those sites. In particular, in spite of the low accessibility at the binding sites of Gcr1 (in YJLWdelta16) and Gcn4 (in iYBR043C), S℘D℘N℘ correctly indicates a high prior probability at those regions.
Figure 5.
S℘D℘N℘ over a Single Sequence Belonging to Multiple Sequence-Sets
The intergenic region iYMR280C belongs to four sequence-sets: Ume6_H2O2Hi, Ume6_YPD, Reb1_H2O2Lo, and Reb1_YPD. The boxes indicate binding sites annotated by Harbison et al. [2]. S℘D℘N℘ for each sequence-set is different although S℘N℘ does not change. S℘D℘N℘ indicates correctly the location of the binding site of the respective TF.
Figure 6.
Transcriptional Complexes Involving Ste12, Tec1, and Dig1
(A) During filamentation, Ste12 forms a complex with Dig1 and Tec1. Tec1 binds DNA, with a sequence specificity for CATTCy. PRIORITY-D℘N℘ finds this motif in all three sequence-sets pulled down by Ste12, Tec1, and Dig1 after the cells are treated with butanol. However, PRIORITY-U℘ misses the functional Tec1 motif in Ste12_BUT14 and Dig1_BUT14. The asterisk indicates that the learned motif is a weak match.
(B) During mating, Ste12 forms two complexes: one with Dig1 and Dig2, and another with Dig1 and Tec1. In either case, it is Ste12 that binds DNA, with a sequence specificity for ATGAAAC. Again, PRIORITY-D℘N℘ finds this motif in all three sequence-sets pulled down by Ste12, Tec1, and Dig1 after the cells are treated with the alpha factor pheromone. Here, PRIORITY-U℘ fails to find the Ste12 motif in Tec1_Alpha. (Figures of the complexes are adapted from Chou et al. [25].)