Figure 1.
Distribution of the Gibbs Free Energy of Stem-Loop Formation
The distribution is calculated from 425 experimentally identified transcriptional terminators in B. subtilis. The dotted curve shows the distribution for E. coli, as calculated from 147 previously collected Rho-independent terminator sequences in this organism [16].
Figure 2.
Distribution of the Length of the Stem in Nucleotides
The distribution is calculated from 425 transcriptional terminators in B. subtilis and 147 previously published Rho-independent terminators in E. coli [16].
Figure 3.
Distribution of Gibbs Free Energy of Stem-Loop Formation, Divided by the Length of the Stem-Loop Structure in Nucleotides
The distribution is calculated from 425 transcriptional terminators in B. subtilis, and 147 previously published Rho-independent terminators in E. coli [16].
Figure 4.
Distribution of the Number of Thymine Residues in the 15 Base Pair T-Stretch following the Stem Loop
The distribution is calculated from 425 transcriptional terminators in B. subtilis, and 147 previously published Rho-independent terminators in E. coli [16].
Figure 5.
Distribution of the Number of Residues in the Loop of Rho-Independent Terminators
The distribution is calculated from 425 transcriptional terminators in B. subtilis and 147 previously collected Rho-independent terminator sequences in E. coli [16].
Figure 6.
Distribution of the Position of B. subtilis Rho-Independent Terminators with Respect to the Stop Codon of the Last Gene in the Operon
The distance between the first nucleotide of the stem-loop and the last nucleotide of the stop codon is shown.
Figure 7.
The yqfSU Operon in B. subtilis Consists of the Two Genes yqfS and yqfU, Separated by the Intervening Gene yqfT, Located on the Opposite Strand
The terminator sequence downstream of yqfT is virtually identical to the complementary sequence on the opposite strand. However, a Northern blotting experiment [27] revealed that the complementary sequence does not act as a transcriptional terminator. Arrows indicate transcription start sites; stem-loops represent transcriptional terminators.
Figure 8.
Sensitivity of Predicting Transcriptional Terminators, Evaluated for 57 Firmicutes and 29 Other Bacterial Species
Firmicutes are shown in dark gray; other bacterial species are shown in light gray. (+), (−), or (0) in front of the organism name denotes that the organism is Gram-positive, Gram-negative, or lacks a cell wall, respectively.
Figure 9.
Average Gibbs Free Energy of Stem-Loop Formation and the Average Number of Thymine Residues in the T-Stretch
These are calculated from the predicted Rho-independent terminators in the 82 bacterial species we consider. Circles represent organisms belonging to the Firmicutes phylum; crosses represent other bacterial species.