Energy transfer leaves fingerprints in cyanine photoswitching behavior
Fig 4
PFA of multi-fluorophore systems undergoing OET.
(A) Two fluorophores, (B) three fluorophores, (C – H) four fluorophores. The photophysical model includes OET leading the OFF state to transition to S0 with a probability of 0.01%. (A – D) The eCDF of photon arrival times. In case of (D), the data is truncated such that only times ≥ 3 s are included to mimic acquisition in the beginning of an experiment. The markers depict simulated results. The analytical expression (solid lines) and fitting procedure are described in section 8 in S1 Text. (E) Probability histogram of photon counts per frame. (F) Probability histogram of ON periods. (G) Probability histogram of OFF periods. (H) Probability histogram of number of ON periods, i.e., ON events. A full set of all corresponding plots is provided in Fig L in S1 Text. Representative fluorescence time traces are provided in Fig M in S1 Text. Representative photophysical statistics of four fluorophores at 3 nm are provided in Fig N in S1 Text.