Energy transfer leaves fingerprints in cyanine photoswitching behavior

doi:10.1371/journal.pcbi.1014322

Energy transfer leaves fingerprints in cyanine photoswitching behavior

Fig 3

Parameters that control the shape of PFA of single Cy5 fluorophores.

(A, B) Altering the photobleaching rate . (C, D) Altering the rates associated with entering the OFF state (, see Table B in S1 Text). (E, F) Altering the rates associated with entering the ON state (, see Table B in S1 Text). (A, C, E) The eCDF of photon arrival times. The markers depict simulated results. The analytical expression (solid lines) and fitting procedure are described in section 8 in S1 Text. (B) Probability histogram of total photon counts during 300 s. (D) Probability histogram of ON periods. (F) Probability histogram of OFF periods. A full set of all corresponding plots is provided in Fig H in S1 Text. For adjusting via thiol concentration, see Fig I in S1 Text.

doi: https://doi.org/10.1371/journal.pcbi.1014322.g003