Energy transfer leaves fingerprints in cyanine photoswitching behavior
Fig 2
Photophysical statistics of a single Cy5 fluorophore with continuous excitation.
Photobleaching is disabled. (A – F) Statistics that are experimentally not available. They can also be predicted (crosses for A – E, line for F) using tools like the limiting distribution of the Markov chain. (A) The probability of occurrence of each photophysical state. (B) The probability of occurrence of each photophysical transition. (C) The lifetime of each photophysical state. (D) The transition lifetime of each photophysical transition. (E) The probability of occupation of each photophysical state. (F) The ePDF of durations of the photophysical state S1 overlaid with an exponential decay of corresponding relaxation time. (G – L) Analyses that are experimentally available. (G) The fluorescence trajectory showing photons per frame over time. (H) The eCDF of photon arrival times. (I) The ePDF of photon counts per frame. (J) The fluorescence correlation curve . (K) The ePDF of OFF periods (consecutive frames with photon count < 10). (L) The ePDF of ON periods (consecutive frames with photon count ≥ 10).