The differentiation of myeloid progenitors is effected by cascading waves of coordinated gene expression that remodel cellular physiology in a characteristic sequence
Fig 3
The identification of genes expressed differentially between the endpoints of the differentiation.
Gene expressed differentially between the endpoints of PUER differentiation (Fig 1A). A–D. Scatter plots of p-value vs. fold change for all the genes. The p-value and fold change thresholds used to identify DEGs (see Methods) are shown as horizontal and vertical dashed lines respectively. Key differentially expressed lineage markers and TFs have been annotated. E–F. Venn diagrams showing the intersection of the different sets of DEGs identified in this analysis. A. Comparison of undifferentiated PUER cells (−48h) with cells treated with OHT for 7 days in GCSF conditions (168h). B. Comparison of PUER cells pre-treated with GCSF for 48 hours (0h) with cells treated with OHT for 7 days in GCSF conditions (168h). C. Comparison of undifferentiated PUER cells (−48h) with those pre-treated with GCSF for 48 hours (0h). D. Comparison of undifferentiated PUER cells (−48h) with those treated with OHT for 7 days in IL3 conditions (168h). E,F. Overlap of DEGs for selected time points for down-regulated (E) and up-regulated (F) genes.