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Single-cell data integration across weakly linked modalities

Fig 1

Overview pipeline of MMIHCL.

(a) The input modality matrices and share linked features. Weighted adjacency cell graphs and are constructed through the akNN module. (b) Linked features are embedded via the hypergraph operator to generate initial embeddings, followed by a Many-to-Many (M-to-M) matching process to obtain the initial matching . (c) The workflow iteratively updates cell embeddings and matching for iterations. Each loop involves , Canonical Correlation Analysis (CCA) [27], and M-to-M matching. The final outputs are the optimized joint embeddings and the 1-to-N matching . (d) Feature similarity is calculated to transition from a constant k in standard kNN to a cell-specific adaptive , yielding the final graph . (e) For a target cell i, local and global message-passing branches generate embeddings and , respectively. Contrastive learning is employed between the two branches to enhance representation robustness, followed by a fusion step to obtain the final learned embedding .

Fig 1

doi: https://doi.org/10.1371/journal.pcbi.1014231.g001