Subunit-specific behavioral modulation of sensory tuning in the visual cortex
Fig 2
Analysis of tuning to stimulus direction and running speed in the mouse visual cortex.
a) Example traces of the calcium imaging data (dF/F) and of mouse running speed obtained from the Allen Brain Observatory (top, dark gray lines and bottom, light gray lines respectively). Gray rectangles mark duration of the stimulus whose orientation and temporal frequency are marked with gray text. b) Left panel: example three-dimensional (running speed - stimulus direction - stimulus temporal frequency) tuning curve of a neuron computed using thresholded dF/F signal (see Methods). Right panel: a two-dimensional (running speed - stimulus direction) tuning curve obtained by averaging the three-dimensional tuning curve on the left across the temporal frequency dimension. c) Distribution of seconds of data per bin in all analyzed datasets. The horizontal white line denotes the average (14 s) and the gray rectangle denotes the interval spanned by 5th and 95th percentiles of the distribution. d) Distributions of the horizontal eye positions pooled across raw recordings (left, gray bar) and averaged within bins of the orientation-speed tuning curves (right, green bar). White lines denote the mean (0 deg) and rectangles denote the interval spanned by 5th and 95th percentiles. Data for each animal was standardized independently via z-scoring. The scale of the plot is symlog (i.e., . The eye position data was not available for all analyzed datasets. e) Left panel: orientation tuning curves of four example neurons. Right panel: running-speed tuning curves of the same neurons as in the left panel (marked with corresponding letter and color). f) Joint orientation-speed tuning curves of four example neurons marked with corresponding letters and colors in, e. g) Example neurons that pass the bootstrap test of velocity modulation. Left column: orientation-speed tuning curves of three example neurons (orange frames). Middle column: distributions of the test statistic of the modulation test (see Methods). Vertical dashed line denotes the significance threshold equal to the 95th percentile of the statistic distribution. Orange and gray triangles denote respectively the values of the test statistic of the original tuning curve and five example bootstrapped samples visualized in the right column. Right column: five example bootstrapped orientation-speed tuning curves. h) Example neurons that do not pass the bootstrap test of velocity modulation. Panel is analogous to g).