A multimodal spatial atlas of transcriptomic, morphological, and electrophysiological cell type densities in the mouse brain
Fig 5
Probabilistic mapping application on t-type densities and validation of mRNA counts globally.
A. Example of a coronal section of the density of the IN_dend_1_ax_3|bAC me-type, which preferentially maps to basket cells related t-types after applying the probabilistic mapping (left) and a heatmap (right) of obtained densities for a selection of brain regions (rows) and a selection of me-type (columns). For clarity, only a subset of me-types and brain regions are shown (including regions of CA1, DG, VIS, SS). B. mRNA counts obtained by multiplying cell types gene expression profiles with their densities (see Methods) in a selection brain regions and a selection of genes for t-types (left) and me-types (middle) and their relative error (right). C. Semilogarithmic plots of mRNA counts summed over regions (left) or genes (right). The top row shows the relative error, defined as (log₁₀ scale), while the bottom row shows the corresponding summed mRNA counts (log₁₀ scale). Blue points correspond to values derived from transcriptomic t-type data (
), and orange points to values obtained from morphological-electrophysiological me-type estimates (
).