Deciphering the cardiac neuron landscape in heart failure patients
Fig 2
Identification of neuronal subsets associated with heart failure.
(a) The heatmap displaying the top five differentially expressed genes in each neuronal cluster. (b) Definition of neuronal subsets. (c) Differences in the frequency of each neuronal subset between heart failure and healthy groups. (d) Comparisons upon the percentage of neuronal subsets between heart failure patients and healthy donors. (e) Overlapping of differential genes in neuronal subsets between our generated data and SCP1303 dataset. Hypergeometric test was used. (f) The enrichment scores of N1-XKR4, N2-OGFRL1, and N4-ALK marker genes in the SCP1303 dataset. (g) The expression of marker genes from N4-ALK, N1-XKR4, and N2-OGFRL1 over neuronal clusters in the dataset of SCP1303. (h) Left Changes in the frequency of neuronal subsets before and after receiving LVAD implantation in patients who did not respond to the treatment. Center Changes in the frequency of neuronal subsets in response to LVAD before and after receiving the treatment. Right Changes in the frequency of neuronal subsets at the statuses of responsive and unresponsive after receiving LVAD implantation. (i) The differential expression of LRRC4C in response to LVAD before and after receiving the implantation. *P < 0.05, **P < 0.01, ***P < 0.001, ns, no significance. In (d) and (i), P value was calculated by Wilcoxon rank-sum test. R or NR state, LVAD responsive or unresponsive status; Post State, after receiving LVAD implantation; R or NR pre, before LVAD implantation for treating individuals with or without response; R or NR post, after LVAD implantation for individuals with or without response.