Online tutorial on survival analysis for biomarker discovery
Fig 3
Gene set enrichment analysis workflow for survival prediction.
The workflow guides trainees through the process of calculating ssGSEA enrichment scores for a specific gene set and stratifying patients based on these scores. The workflow stratifies patients based on ssGSEA scores and visualizes Kaplan-Meier survival curves, revealing the prognostic impact of biological pathways. Users can experiment interactively with different gene sets to identify biologically meaningful predictors of survival. We use this workflow to support learners in understanding how pathway-level features may provide more robust and interpretable survival predictors than individual genes.