< Back to Article

napariTFM: An open-source tool for traction force microscopy and monolayer stress microscopy

Fig 5

Validation of napariTFM against experimental data, previously published in eLife [37].

(A) Single-cell analysis showing displacement fields (left column), traction force fields (second column), and stress tensor components (third column) and (right column). Top row: original analysis from Ruppel et al., eLife 2023. Bottom row: reanalysis using napariTFM. Color maps indicate displacement magnitude in μm, traction force magnitude in Pa, and stress components in mN/m. (B) Population-averaged fields across 29 cells. Top row: averages from original analysis. Bottom row: averages from napariTFM reanalysis. Layout as in (A). (C) Quantitative comparison of analysis outputs. Box plots show distributions of average displacement magnitude (left), average traction force (second from left), average stress (second from right), and average stress (right). For each metric, three conditions are shown: Original (original published analysis), napariTFM (reanalysis), and Difference. Horizontal dashed line at zero indicates no difference between methods.

doi: https://doi.org/10.1371/journal.pcbi.1014045.g005