napariTFM: An open-source tool for traction force microscopy and monolayer stress microscopy
Fig 3
Validation of displacement analysis and traction force calculation.
(A) Displacement field validation across three scenarios (low, mid, high displacement magnitudes). Top row shows ground truth displacement fields, bottom row shows calculated results using napariTFM’s TV-L1 optical flow algorithm. Vector overlays indicate displacement direction and magnitude, with color maps showing displacement magnitude in pixels. (B) Displacement analysis metrics. Left: correlation coefficients between calculated and ground truth displacement fields. Right: relative error as a function of ground truth displacement magnitude, showing systematic bias at different displacement scales. (C) Traction force validation using FTTC algorithm across three force magnitude scenarios. Top row shows ground truth traction fields, bottom row shows calculated results. Color maps indicate traction force magnitude in kPa, with vector overlays showing force direction. (D) Traction force analysis metrics. From left to right: correlation coefficients between calculated and ground truth traction forces, deviation of traction magnitude (DTM), traction in surrounding regions (DTPS) and deviation of traction angle (DTA).