napariTFM: An open-source tool for traction force microscopy and monolayer stress microscopy
Fig 1
napariTFM workflow and data structure.
Schematic overview of the napariTFM analysis pipeline showing the flow from raw input data (beads, reference, and optional cell images) through preprocessing, displacement analysis using TV-L1 optical flow, force calculation via FTTC (Fourier Transform Traction Cytometry), and stress analysis using MSM (Monolayer Stress Microscopy). Orange boxes indicate input data, blue boxes show analysis steps and their internal data structures, and green boxes indicate output files generated at each step.